trans-4-Hydroxy-L-proline-7-amido-4-methylcoumarin hydrochloride
trans-4-Hydroxy-L-proline-7-amido-4-methylcoumarin hydrochloride (CAS: 77471-43-3) is a fluorogenic enzyme substrate with the empirical formula C15H16N2O4 · HCl and a molecular weight of 324.76 g/mol. This compound is primarily utilised in biochemical research for the assay of enzyme activity. Stored at -20°C, it serves as a valuable reagent for scientists investigating enzymatic processes and developing diagnostic tools.
Enzyme Activity Assays
This compound functions as a fluorogenic substrate, releasing a fluorescent signal upon enzymatic cleavage. This property makes it ideal for quantifying the activity of specific enzymes in research and diagnostic applications.
Biochemical Research
As a specialised biochemical reagent, it aids researchers in studying enzyme kinetics, inhibitor screening, and understanding complex biological pathways.
Fluorogenic Probes
Its coumarin-based fluorophore allows for sensitive detection and measurement, making it a useful tool in various biochemical and cellular analysis techniques.
| Molecular weight | 324.76 |
|---|---|
| Empirical formula | C15H16N2O4 · HCl |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is trans-4-Hydroxy-L-proline-7-amido-4-methylcoumarin hydrochloride used for?
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This compound is primarily used as a fluorogenic enzyme substrate for the assay of enzyme activity in biochemical research and diagnostic applications.
What are the CAS number and formula for this product?
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The CAS number is 77471-43-3 and the empirical formula is C15H16N2O4 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research and laboratory use. The specific assay and purity details are available on the product's Certificate of Analysis. TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should trans-4-Hydroxy-L-proline-7-amido-4-methylcoumarin hydrochloride be handled and stored?
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Handle with appropriate personal protective equipment including eyeshields and gloves. A type N95 (US) or P1 (EN143) respirator filter is recommended. Store at -20°C as indicated by the safety data. This compound is classified under WGK Germany 3.
Is this product controlled or scheduled?
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The provided data does not indicate that this compound is a controlled or scheduled substance.
How can I request a quote or sample?
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Please contact our customer service team or visit the product page on our website to request a quote or a sample for evaluation.
How is this chemical packed, shipped, and exported?
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Tech Serve Solutions professionally packs and ships fine chemicals, pharmaceutical intermediates, and laboratory reagents globally, adhering to international shipping regulations.
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