Rhodamine B isothiocyanate
Rhodamine B isothiocyanate (CAS: 36877-69-7), with the molecular formula C29H30ClN3O3S, is a high-purity fluorescent labelling reagent. This powder compound offers excellent solubility in methanol and is specifically suited for protein labelling. Its distinct absorption and fluorescence spectra, with absorption maximum at 555 nm and excitation/emission maxima of 540 nm and 573 nm respectively in ethanol, make it ideal for various biological and chemical detection applications.
- IUPAC
- RBITC
Protein Labeling
Rhodamine B isothiocyanate is employed for the covalent conjugation of fluorescent labels to proteins. This enables sensitive detection and tracking of proteins in various biological assays.
Fluorescent Probes
As a fluorescent probe, it can be utilised in microscopy and flow cytometry to visualise cellular structures or monitor biological processes requiring fluorescent tagging.
Biochemical Research
This reagent serves as a valuable tool in biochemical research for developing assays and investigating biological interactions that benefit from fluorescent readouts.
Histology and Hematology
Its staining properties make it suitable for applications in histology and hematology, aiding in the differentiation and visualisation of cells and tissues.
| Molecular weight | 536.08 |
|---|---|
| Empirical formula | C29H30ClN3O3S |
| Form | powder |
| Solubility | methanol: soluble10 mg/mL |
| Storage temperature | 2-8°C |
| Suitability | suitable for protein labeling |
Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
- H334May cause allergy or asthma symptoms if inhaled
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
- P342
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Transport (UN / ADR) | UN 3335 |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/21/22-36/37/38-42 |
| Safety statements (S) | 22-26-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 555 nm |
|---|---|
| Fluorescence | λex 540 nm; λem 573 nm in ethanol |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Rhodamine B isothiocyanate used for?
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Rhodamine B isothiocyanate is primarily used for protein labelling and as a fluorescent probe in various biological and chemical detection applications, including microscopy, flow cytometry, and biochemical research. It is also suitable for applications in histology and hematology.
What is the CAS number and molecular formula for Rhodamine B isothiocyanate?
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The CAS number for Rhodamine B isothiocyanate is 36877-69-7, and its molecular formula is C29H30ClN3O3S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Rhodamine B isothiocyanate as a powder, suitable for protein labeling, with specified optical properties. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling considerations for Rhodamine B isothiocyanate?
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Rhodamine B isothiocyanate is classified as Danger, with hazards including harmful if swallowed, in contact with skin, or inhaled (H302 + H312 + H332), causes skin irritation (H315), causes serious eye irritation (H319), and may cause allergy or asthma symptoms or breathing difficulties if inhaled (H334, H335). Appropriate personal protective equipment, including a dust mask (N95), eyeshields, face shields, and gloves, must be used. Handle in a well-ventilated area.
How is Rhodamine B isothiocyanate shipped and exported?
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Rhodamine B isothiocyanate, UN 3335, is shipped and exported according to relevant transportation regulations. Storage at 2-8°C is recommended.
How can I request a sample or quote for Rhodamine B isothiocyanate?
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To request a sample or obtain a quote for Rhodamine B isothiocyanate, please contact our sales department via the website or by phone.
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