Rhodamine B
Rhodamine B (CAS: 81-88-9), with the chemical formula C28H31ClN2O3 and a molecular weight of 479.01 g/mol, is a vibrant fluorescent dye. Its intense pink to red fluorescence makes it valuable in various scientific and industrial applications. It is widely used as a fluorescent tracer, a biological stain, and in the formulation of inks and other colourants. TSS supplies Rhodamine B as a high-purity powder.
- IUPAC
- Basic Violet 10,Brilliant Pink B,Rhodamine O,Tetraethylrhodamine
- Synonyms
- Basic Violet 10Brilliant Pink BRhodamine OTetraethylrhodamine

Fluorescent Tracer
Rhodamine B is an effective fluorescent tracer for hydrological studies, allowing for the tracking of water flow in rivers, groundwater, and oceanic currents. Its high fluorescence quantum yield ensures reliable detection even at low concentrations.
Biological Staining
In histology and cell biology, Rhodamine B serves as a fluorescent stain to visualise cellular structures and components. It is particularly useful for staining cytoplasm and identifying lysosomal activity due to its fluorescence properties.
Dye and Colourant Applications
The brilliant pinkish-red hue of Rhodamine B makes it suitable for use in the formulation of inks, laser dyes, and as a colourant in non-food applications where its strong fluorescence is desired.
Luminescent Detection
As a luminescent compound, Rhodamine B is employed in various detection assays and as a fluorescent marker in research settings. Its spectral properties allow for its use in fluorescence microscopy and spectroscopy.
| Molecular weight | 479.01 |
|---|---|
| Empirical formula | C28H31ClN2O3 |
| Assay | ≥95% (HPLC) |
| Form | powder |
| Solubility | H2O: soluble1 mg/mL |
| Melting point | 210-211 (dec.)(lit.) |


Hazard statements
- H302Harmful if swallowed
- H318Causes serious eye damage
- H412Harmful to aquatic life with long-lasting effects
Precautionary statements
- P273Avoid release to the environment
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-41-52/53 |
| Safety statements (S) | 26-39 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 45170 |
|---|---|
| Absorption | extinction/542-554 nm, methanol 104500-115800 |
| Fluorescence | λex 554 nm; λem 627 nm (Acidic EtOH) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Rhodamine B used for?
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Rhodamine B is primarily used as a fluorescent tracer in hydrological studies, a biological stain in histology and cell biology, and as a vibrant colourant in inks and other non-food applications. It is also utilised for luminescent detection in research.
What are the CAS number and chemical formula for Rhodamine B?
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The CAS number for Rhodamine B is 81-88-9, and its chemical formula is C28H31ClN2O3.
What grade and purity does Tech Serve Solutions supply for Rhodamine B?
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Tech Serve Solutions supplies Rhodamine B with an assay of ≥95% (HPLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should Rhodamine B be handled safely?
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Rhodamine B is classified as dangerous. It is harmful if swallowed (H302), causes serious eye damage (H318), and is harmful to aquatic life with long-lasting effects (H412). When handling, use a dust mask (type N95 US), eye shields, and gloves. Ensure good ventilation and avoid release into the environment (P273).
How is Rhodamine B packed, shipped, and exported?
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Rhodamine B is supplied as a powder. As a global supplier, Tech Serve Solutions adheres to all necessary regulations for the safe packing, shipping, and export of chemicals worldwide.
How can I request a sample or quote for Rhodamine B?
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To request a sample or a quote for Rhodamine B, please contact the Tech Serve Solutions sales team through our website or by phone. Provide your contact details and the quantity required.
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