Resorufin ethyl ether
Resorufin ethyl ether (CAS: 5725-91-7), with the molecular formula C14H11NO3 and a molecular weight of 241.24 g/mol, is a fluorescent compound. It serves as a valuable substrate in biochemical research, particularly for the detection and quantitation of enzyme activities. Its fluorescent properties make it useful for studying drug and xenobiotic metabolism and cell signaling pathways.
- IUPAC
- 7-Ethoxy-3H-phenoxazin-3-one,Ethoxyresorufin,O7-Ethylresorufin
- Synonyms
- 7-Ethoxy-3H-phenoxazin-3-oneEthoxyresorufinO7-Ethylresorufin

Enzyme Substrate
Resorufin ethyl ether is utilized as a substrate for various enzymes, enabling the study of enzyme kinetics and activity. Its conversion leads to a fluorescent product, facilitating sensitive detection methods.
Fluorescence Assays
This compound is suitable for fluorescence-based assays due to its distinct excitation and emission wavelengths. It is employed in research for detecting and quantifying biological processes involving specific enzymes.
Drug Metabolism Studies
Its role as a substrate in pathways like Cytochrome P450 metabolism makes it relevant for studying drug and xenobiotic interactions and their metabolic fates within biological systems.
Cell Signaling Research
Resorufin ethyl ether can be applied in cell biology to investigate cell signaling pathways and enzyme functions within cellular environments, particularly those involving esterase or oxidase activities.
| Molecular weight | 241.24 |
|---|---|
| Empirical formula | C14H11NO3 |
| Assay | ≥95% (UV) |
| Solubility | DMF: solubleDMSO: solublealcohols: soluble |
| Storage temperature | 2-8°C |
| Suitability | suitable for fluorescence |
| Melting point | 223-225 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 464 nm; λem 540 nm in methanol(lit.)λex 571 nm; λem 585 nm in dealkylase(lit.) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Resorufin ethyl ether used for?
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Resorufin ethyl ether is used as a fluorescent enzyme substrate for detecting and quantifying enzyme activity, particularly in studies of drug metabolism, xenobiotic metabolism, and cell signaling pathways.
What are the CAS number and chemical formula for Resorufin ethyl ether?
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The CAS number is 5725-91-7, and the empirical formula is C14H11NO3.
What grade and purity does Tech Serve Solutions supply for Resorufin ethyl ether?
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Tech Serve Solutions supplies Resorufin ethyl ether with an assay of ≥95% (UV). This product is intended for research purposes and is not represented as USP, BP, EP, or pharmacopoeia grade.
How should Resorufin ethyl ether be handled and stored?
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Handle Resorufin ethyl ether with appropriate personal protective equipment, including eyeshields and gloves. A type N95 respirator filter is recommended. Store the compound at 2-8°C to maintain its stability.
Is Resorufin ethyl ether a controlled substance?
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The provided data does not indicate that Resorufin ethyl ether is a controlled substance.
How are products like Resorufin ethyl ether packed, shipped, and exported by TSS?
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Tech Serve Solutions carefully packs and ships chemicals globally, adhering to all relevant regulations for safe and secure export. Specific packaging details are available upon request.
How can I request a quote or sample for Resorufin ethyl ether?
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To request a quote or sample for Resorufin ethyl ether, please contact our sales department through the TSS website or by phone.
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