Resorufin acetate
Resorufin acetate (CAS: 1152-14-3; C14H9NO4; Molar Mass: 255.23 g/mol) is a valuable fluorescent biochemical reagent. It serves as a fluorogenic substrate for various hydrolytic enzymes, including cellulases and chymotrypsin. Its ability to emit fluorescence upon enzymatic cleavage makes it a key compound for detection and analysis in biochemical research and diagnostic applications.
Fluorogenic Enzyme Substrate
Resorufin acetate is utilised as a sensitive fluorogenic substrate for the detection and quantification of esterase activity. Upon hydrolysis by enzymes like chymotrypsin, it releases resorufin, a fluorescent molecule whose emission can be measured.
Biochemical Research
It is employed in various biochemical assays to study enzyme kinetics and function. The compound is particularly useful for researchers investigating enzymes involved in cellular processes where esterase activity is significant.
Fluorescence Microscopy and Spectroscopy
Due to its fluorescent properties, resorufin acetate and its hydrolysis product are suitable for applications in fluorescence microscopy and spectroscopy, aiding in cellular imaging and biochemical analysis.
| Molecular weight | 255.23 |
|---|---|
| Empirical formula | C14H9NO4 |
| Assay | ≥98.0% (TLC) |
| Solubility | DMF: solubleDMSO: solublealcohols: soluble |
| Storage temperature | 2-8°C |
| Application | suitable as fluorogenic substrate for hydrolytic enzymes (cellulases, chymotrypsin) |
| Suitability | suitable for fluorescence |
| Melting point | 223 °C(lit.) |
Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
- H332Harmful if inhaled
Precautionary statements
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/22-36/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 500 nm; λem 593 nm in 0.1 M phosphate pH 8.0 (chymotrypsin)λex 540 nm; λem 590 nm in DMSOλex 571 nm; λem 584 nm (Reaction product) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Resorufin acetate used for?
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Resorufin acetate is used as a fluorogenic substrate for hydrolytic enzymes, including cellulases and chymotrypsin, and is valuable in biochemical research, fluorescence microscopy, and spectroscopy.
What is the CAS number and chemical formula for Resorufin acetate?
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The CAS number for Resorufin acetate is 1152-14-3, and its chemical formula is C14H9NO4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Resorufin acetate with an assay of ≥98.0% (TLC). We do not represent this product as meeting USP, BP, or EP pharmacopoeia standards.
How should Resorufin acetate be handled safely?
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Resorufin acetate is classified as harmful if swallowed, causes skin irritation, causes serious eye irritation, and harmful if inhaled (H302-H315-H319-H332). Appropriate personal protective equipment including a dust mask (N95), eyeshields, and gloves should be worn. Handle in a well-ventilated area.
How is Resorufin acetate packaged and shipped?
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Resorufin acetate is typically stored at 2-8°C. We export fine chemicals globally and ensure appropriate packaging for safe transit.
How can I request a quote or sample?
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Please contact the Tech Serve Solutions sales team via our website or direct inquiry to request a quote or sample of Resorufin acetate.
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