Reactive Orange 16
Reactive Orange 16 (CAS: 12225-83-1) is a vibrant organic dye with the molecular formula C20H17N3Na2O11S3 and a molecular weight of 617.54 g/mol. Known also as Remazol Brilliant Orange 3R, this compound is widely utilised as a stain and dye in various scientific disciplines. Its specific absorption characteristics make it valuable in applications within cell biology, hematology, and histology for differential staining and visualisation.
- IUPAC
- Remazol Brilliant Orange 3R
- Synonyms
- Remazol Brilliant Orange 3R

Histological Staining
Reactive Orange 16 serves as a crucial dye in histological preparations. It aids in the differentiation and visualisation of cellular structures, contributing to accurate tissue analysis in research and diagnostics.
Cell Biology Research
Within cell biology, this dye is employed for staining various cellular components. Its spectral properties can be exploited for specific labelling techniques or as a counterstain in complex assays.
Hematology Applications
Reactive Orange 16 finds application in hematology for the staining of blood cells and tissues. This facilitates the identification and classification of different cell types and morphological abnormalities.
Dye & Stain Manufacturing
As a versatile dye, it can be a key intermediate or component in the formulation of specialised staining solutions and diagnostic reagents for laboratory use.
| Molecular weight | 617.54 |
|---|---|
| Empirical formula | C20H17N3Na2O11S3 |
| Melting point | >300 °C(lit.) |

Hazard statements
- H315Causes skin irritation
- H317May cause an allergic skin reaction
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 36/37/38-42/43 |
| Safety statements (S) | 26-37/39 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 17757 |
|---|---|
| Absorption | λmax 388 nmλmax 494 nm (2nd) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Reactive Orange 16 used for?
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Reactive Orange 16 is primarily used as a stain and dye in scientific applications, particularly within cell biology, hematology, and histology for differential staining and visualisation of cellular and tissue structures.
What is the CAS number and chemical formula for Reactive Orange 16?
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The CAS number for Reactive Orange 16 is 12225-83-1, and its molecular formula is C20H17N3Na2O11S3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Reactive Orange 16 as a reagent/technical grade product. We do not represent this product as meeting USP, BP, or EP pharmacopoeial standards. Purity information is available upon request.
How should Reactive Orange 16 be handled safely?
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Reactive Orange 16 is classified with Warning signal word and poses hazards including skin irritation (H315), allergic skin reaction (H317), eye irritation (H319), and respiratory irritation (H335). Appropriate personal protective equipment, including dust masks (N95), eyeshields, face shields, and gloves, must be worn during handling. Work in a well-ventilated area or use local exhaust ventilation.
Is Reactive Orange 16 a controlled substance?
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The provided data does not indicate that Reactive Orange 16 is a controlled substance.
How is Reactive Orange 16 packed and exported?
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Reactive Orange 16 is available for global export, packed according to standard chemical shipping regulations to ensure safe transit and compliance with international transport laws.
How can I request a sample or quote for Reactive Orange 16?
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To request a sample or a quote for Reactive Orange 16, please contact our sales department via the website or direct email, providing your specific requirements.
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