Phenylmethanesulfonyl fluoride

CAS 329-98-6C7H7FO2SBiochemicals & Reagents

Phenylmethanesulfonyl fluoride (CAS: 329-98-6, C7H7FO2S), a PREMIUM grade chemical with a molecular weight of 174.19 g/mol, is a potent inhibitor of serine proteases like trypsin and chymotrypsin. It also exhibits inhibitory activity against cysteine proteases and mammalian acetylcholinesterase. While less potent than DFP, it serves as a valuable reagent in biochemical research and diagnostics, particularly in enzyme studies and cell biology.

IUPAC: α-Toluenesulfonyl fluoride,Benzylsulfonyl fluoride,PMSF,Phenylmethylsulfonyl fluoride

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Phenylmethanesulfonyl fluoride (C7H7FO2S) — chemical structure, CAS 329-98-6; Life Science, fine chemical supplied by Tech Serve Solutions

Bioactive Small MoleculesBiochemicals and ReagentsCarboxypeptidase YCell BiologyCore BioreagentsCysteine Protease InhibitorsD to KElastaseleukocyteEnzyme Inhibitors

01 /Applications

Enzyme Inhibition Studies

Phenylmethanesulfonyl fluoride is widely used to inhibit serine proteases such as trypsin and chymotrypsin in various biochemical assays and purification procedures. Its activity extends to cysteine proteases and mammalian acetylcholinesterase, making it a versatile tool for enzyme characterisation.

Biochemical Reagent

As a bioactive small molecule, it functions as a critical reagent in cell biology research. It is employed to modulate enzymatic activity in complex biological systems, aiding in the understanding of cellular processes and signalling pathways.

Proteomics Research

This compound is utilised in proteomics to prevent protein degradation by endogenous proteases during sample preparation. Its ability to inhibit a broad spectrum of proteolytic enzymes ensures the integrity of protein samples for subsequent analysis.

02 /Properties

Molecular weight	174.19
Empirical formula	C7H7FO2S
Assay	≥98.5% (GC)
Density	0.797 g/mL at 20 °C
Solubility	dry solvents (ethanol, methanol, and 2-propanol): soluble200 mM (Stock solution are stable for months at 4°C.)H2O: unstable
Concentration	~0.1 M in ethanol (T)
Storage temperature	2-8°C
Application	Inhibits serine proteases such as trypsin and chymotrypsin. Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase. Not as effective or as toxic as DFP. Effective concentration 0.1-1 mM. Half-life

03 /Safety & handling

GHS hazard pictogram: Corrosive (GHS05) — Corrosive

GHS hazard pictogram: Toxic (GHS06) — Toxic

Danger

Hazard statements

H301Toxic if swallowed
H314Causes severe skin burns and eye damage

Precautionary statements

P280Wear protective gloves, clothing and eye/face protection
P305IF IN EYES
P310Immediately call a POISON CENTER or doctor

Protective equipment	Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
Transport (UN / ADR)	UN 2928 6.1/PG 2
Water hazard class (WGK, DE)	3
Hazard codes (EU)	T
Risk statements (R)	25-34
Safety statements (S)	26-36/37/39-45

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

329-98-6

MDL number

MFCD00007424

PubChem substance

24898863

Beilstein registry

2088311

05 /Additional specifications

Quality Level	PREMIUM

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Phenylmethanesulfonyl fluoride used for?

Phenylmethanesulfonyl fluoride (PMSF) is primarily used as an inhibitor of serine proteases, such as trypsin and chymotrypsin, as well as cysteine proteases and mammalian acetylcholinesterase in biochemical research and diagnostics.

What is the CAS number and molecular formula for Phenylmethanesulfonyl fluoride?

The CAS number for Phenylmethanesulfonyl fluoride is 329-98-6, and its molecular formula is C7H7FO2S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Phenylmethanesulfonyl fluoride at a PREMIUM quality level with an assay of ≥98.5% (GC). TSS does not represent this product as USP, BP, or EP grade.

How should Phenylmethanesulfonyl fluoride be handled safely?

Phenylmethanesulfonyl fluoride is a dangerous substance requiring strict handling precautions. It is toxic if swallowed and causes severe skin burns and eye damage (H301-H314). Appropriate personal protective equipment, including eyeshields, face shields, chemical-resistant gloves, and respiratory protection (type P3 or N100), must be used in a well-ventilated area or fume hood.

How is Phenylmethanesulfonyl fluoride shipped and exported?

Phenylmethanesulfonyl fluoride is shipped internationally by Tech Serve Solutions under UN 2928, Hazard Class 6.1 (Toxic substances), Packing Group II. We adhere to all regulations for the safe transport and export of hazardous chemicals.

How can I request a sample or quote for Phenylmethanesulfonyl fluoride?

To request a sample or a quote for Phenylmethanesulfonyl fluoride, please contact our sales department via the website or by phone, providing your specific requirements and shipping details.