Oil Red O
Oil Red O (CAS: 1320-06-5), a lipophilic diazo dye, presents as a reddish-brown powder. With the molecular formula C26H24N4O and a molecular weight of 408.49 g/mol, it is highly valued for its specific affinity to lipids and fat-soluble substances. Its primary applications are in biological staining, particularly for the detection and visualisation of neutral triglycerides, lipoproteins, and other lipids within cells and tissues, making it indispensable in histology and cell biology research.
- IUPAC
- 1-([4-(Xylylazo)xylyl]azo)-2-naphthol,1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol,Solvent Red 27
- Synonyms
- Solvent Red 271-([4-(Xylylazo)xylyl]azo)-2-naphthol1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol
Lipid Staining in Histology
Oil Red O is widely employed to stain neutral lipids and triglycerides in tissue sections. The dye selectively colours these hydrophobic molecules, allowing for their clear visualisation and localisation under microscopy.
Cell Biology Research
This stain is crucial for studying cellular lipid metabolism and fat accumulation in various cell types. It aids in identifying adipocytes and monitoring lipid droplet formation in response to experimental conditions.
Electrophoresis Applications
Certified as suitable for electrophoresis, Oil Red O can be used in conjunction with separation techniques to identify and visualise lipid-rich components within biological samples following electrophoretic separation.
| Molecular weight | 408.49 |
|---|---|
| Empirical formula | C26H24N4O |
| Grade | certified by the Biological Stain Commission |
| Form | powder |
| Solubility | chloroform/ethanol (1:1): soluble1 mg/mL |
| Concentration | 0.5% in isopropanol |
| Suitability | suitable for electrophoresis |
| Melting point | 120 °C (dec.)(lit.) |
| Composition | Dye content, ≥75% |
Precautionary statements
- P210Keep away from heat, sparks and open flames. No smoking
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 26125 |
|---|---|
| Absorption | λmax 359 nmλmax 518 nm (2nd) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Oil Red O used for?
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Oil Red O is primarily used as a lipophilic stain for the visualisation and localisation of neutral triglycerides, lipoproteins, and other lipids in cells and tissues, particularly in histological and cell biology applications.
What are the CAS number and formula for Oil Red O?
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The CAS number for Oil Red O is 1320-06-5, and its molecular formula is C26H24N4O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Oil Red O certified by the Biological Stain Commission, with a dye content of ≥75%. It is suitable for electrophoresis. TSS does not represent this product as USP, BP, or EP grade.
How should Oil Red O be handled safely?
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When handling Oil Red O, ensure adequate personal protective equipment, including eyeshields, gloves, and a P1 (EN143) or N95 (US) respirator filter. Avoid breathing dust and ensure good ventilation. Refer to the Safety Data Sheet for full guidance.
How is Oil Red O packed and shipped?
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Oil Red O is typically supplied as a powder and is carefully packaged to ensure its integrity during transport. We export globally, adhering to all relevant shipping regulations for chemical substances.
How can I request a quote or sample for Oil Red O?
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To request a quote or a sample of Oil Red O, please contact our sales team through our website or by phone, providing your specific requirements and shipping details.
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