o-Dianisidine dihydrochloride
o-Dianisidine dihydrochloride (CAS: 20325-40-0) is a white to pinkish-white wet powder with a molecular weight of 317.21 g/mol and the empirical formula C14H16N2O2 · 2HCl. This compound is primarily utilised as a chromogenic substrate for enzymatic assays, particularly in the detection of peroxidase activity. Its reliable performance makes it a valuable reagent in biochemical research and diagnostic applications requiring sensitive colorimetric detection.
- IUPAC
- o-Dianisidine dihydrochloride
Peroxidase Substrate
o-Dianisidine dihydrochloride serves as a key chromogenic substrate for detecting peroxidase activity in various biochemical and diagnostic assays. It produces a coloured product upon enzymatic oxidation, enabling quantitative measurement.
Enzyme Assays
This compound is widely employed in enzyme-linked immunosorbent assays (ELISA) and other in-vitro diagnostic tests. Its sensitivity allows for the detection of low levels of enzyme activity.
Research Reagent
In research laboratories, o-Dianisidine dihydrochloride is used for developing and validating new analytical methods and for fundamental studies in enzymology and biochemistry.
| Molecular weight | 317.21 |
|---|---|
| Empirical formula | C14H16N2O2 · 2HCl |
| Assay | 99 % Min. (HPLC) |
| Solubility | H2O: soluble25 mg/mL |
| Storage temperature | 2-8°C |
| Melting point | 268 °C (dec.)(lit.) |
Hazard statements
- H302Harmful if swallowed
- H350May cause cancer
Precautionary statements
- P201Obtain special instructions before use
- P308IF exposed or concerned
| Protective equipment | Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 45-22 |
| Safety statements (S) | 53-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Description | White to pinkish white wet powder |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is o-Dianisidine dihydrochloride used for?
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o-Dianisidine dihydrochloride is primarily used as a chromogenic substrate for peroxidase activity in biochemical assays and diagnostic tests.
What are the CAS number and formula for o-Dianisidine dihydrochloride?
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The CAS number is 20325-40-0 and the empirical formula is C14H16N2O2 · 2HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies o-Dianisidine dihydrochloride with a minimum assay of 99% (HPLC). We do not represent this product as USP, BP, or EP grade.
How should o-Dianisidine dihydrochloride be handled safely?
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o-Dianisidine dihydrochloride is harmful if swallowed and suspected of causing cancer. Handle with appropriate personal protective equipment, including eyeshields, gloves, and respiratory protection (N100 US/P3 EN143). Ensure adequate ventilation and avoid dust formation. Consult the Safety Data Sheet for full details.
How is o-Dianisidine dihydrochloride packed and shipped?
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This chemical is typically supplied as a wet powder and shipped according to standard hazardous material regulations. Please contact TSS for specific packaging and export details.
How can I request a sample or quote for o-Dianisidine dihydrochloride?
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To request a sample or a quote, please contact the Tech Serve Solutions sales team via our website or direct email, providing your contact information and the quantity required.
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