Ninhydrin
Ninhydrin (CAS 485-47-2), with the empirical formula C9H6O4 and a molecular weight of 178.14 g/mol, is a versatile organic compound. It is primarily recognised for its utility in analytical chemistry, specifically for the detection and quantification of free amino groups in amino acids, peptides, and proteins. This reagent is indispensable for chromatographic and spectrophotometric analyses, contributing to its significance in biochemical research and diagnostics.
- IUPAC
- 1,2,3-Indantrione monohydrate,2,2-Dihydroxy-1,3-indanedione,Trioxohydrindene monohydrate
- Synonyms
- 1,2,3-Indantrione monohydrate2,2-Dihydroxy-1,3-indanedioneTrioxohydrindene monohydrate
Amino Acid Analysis
Ninhydrin is widely employed as a derivatization reagent for the detection of amino acids. It reacts with primary and secondary amines to produce a characteristic purple colour (Ruhemann's purple), allowing for sensitive spectrophotometric quantification.
Peptide and Protein Characterization
In addition to free amino acids, Ninhydrin is used to detect amino groups in peptides and proteins. This application is crucial for determining protein concentration and for analysing peptide sequences.
Biochemical Research
As a fundamental biochemical reagent, Ninhydrin supports various research endeavours involving biological molecules, facilitating the study of metabolic pathways and protein structures.
Histology and Hematology
Ninhydrin can be utilised as a stain in histological and hematological preparations to visualise amine-containing structures within cells and tissues.
| Molecular weight | 178.14 |
|---|---|
| Empirical formula | C9H6O4 |
| Assay | ≥95.0% (UV) |
| Grade | SAJ special grade |
| Application | Used for the detection of free amino groups in amino acids, peptides and proteins. |
| Suitability | suitable for amino acid analysis |
| Melting point | 250 °C (dec.)(lit.) |
Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-36/37/38 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Availability | available only in Japan |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Ninhydrin used for?
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Ninhydrin is primarily used for the detection of free amino groups in amino acids, peptides, and proteins, and can also be used as a stain in histology and hematology.
What are the CAS number and formula for Ninhydrin?
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The CAS number for Ninhydrin is 485-47-2, and its empirical formula is C9H6O4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Ninhydrin in SAJ special grade with an assay of ≥95.0% (UV), suitable for amino acid analysis. We do not represent this product as USP, BP, or EP grade.
How should Ninhydrin be handled safely?
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Ninhydrin is a warning substance that may cause harm if swallowed, and causes skin and eye irritation, as well as respiratory irritation. Handle with appropriate personal protective equipment including a dust mask type N95, eyeshields, and gloves. Ensure good ventilation. Refer to the Safety Data Sheet for detailed handling instructions.
Is Ninhydrin available for export?
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Ninhydrin is available only in Japan as per the product data. Tech Serve Solutions can provide export quotations upon request for regions where it is permissible.
How can I request a quote or sample for Ninhydrin?
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To request a quote or sample for Ninhydrin, please contact our sales team via the website or direct email, specifying the product CAS number (485-47-2) and your requirements.
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