N,O-Bis(trimethylsilyl)trifluoroacetamide

CAS 25561-30-2CF3C[=NSi(CH3)3]OSi(CH3)3Biochemicals & Reagents

N,O-Bis(trimethylsilyl)trifluoroacetamide (CAS 25561-30-2) is a silylating agent with the molecular formula C7H16F3NOSi2 and a molar mass of 257.40 g/mol. It is primarily utilised in analytical chemistry as a derivatization reagent. This compound effectively increases the volatility and thermal stability of various analytes, making them suitable for gas chromatography (GC) analysis. Its ability to react with active hydrogen atoms is key to its application.

IUPAC: BSTFA
Synonyms: CF3C[=NSi(CH3)3]OSi(CH3)3BSTFA

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N,O-Bis(trimethylsilyl)trifluoroacetamide (CF3C[=NSi(CH3)3]OSi(CH3)3) — chemical structure, CAS 25561-30-2; Life Science, fine chemical supplied by Tech Serve Solutions

Analytical ReagentsAnalytical/ChromatographyBiochemicals and ReagentsDerivatization ReagentsDerivatization Reagents GCDerivatization agentsReagents for SilylationSilylation Reagents More...

01 /Applications

Gas Chromatography Derivatization

N,O-Bis(trimethylsilyl)trifluoroacetamide serves as a potent derivatization reagent for gas chromatography. It reacts with active hydrogen atoms in organic molecules, converting them into more volatile and thermally stable trimethylsilyl (TMS) ethers or esters. This facilitates improved separation and detection of compounds that might otherwise be unsuitable for direct GC analysis.

Analytical Chemistry Reagent

As a specialized reagent, this compound is employed to enhance the analytical performance of various samples. By forming TMS derivatives, it aids in the identification and quantification of complex organic compounds in diverse matrices, including pharmaceutical, environmental, and biological samples.

Silylation Agent

This chemical is a versatile silylating agent used to introduce trimethylsilyl groups onto functional groups containing active hydrogen. The resultant silylated compounds often exhibit increased volatility, improved chromatographic properties, and enhanced stability.

02 /Properties

Molecular weight	257.40
Linear formula	CF3C[=NSi(CH3)3]OSi(CH3)3
Assay	≥99%
Grade	for GC derivatization
Boiling point	45-50 °C/14 mmHg(lit.)
Density	0.969 g/mL at 25 °C(lit.)
Refractive index	n20/D 1.384(lit.)
Storage temperature	2-8°C
Contains / stabiliser	1% trimethylchlorosilane as additive

03 /Safety & handling

GHS hazard pictogram: Flammable (GHS02) — Flammable

GHS hazard pictogram: Corrosive (GHS05) — Corrosive

Danger

Hazard statements

H226Flammable liquid and vapour
H314Causes severe skin burns and eye damage

Precautionary statements

P280Wear protective gloves, clothing and eye/face protection
P305IF IN EYES
P310Immediately call a POISON CENTER or doctor

Protective equipment	Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
Flash point	24 °C / 75.2 °F
Transport (UN / ADR)	UN 2924 8(3) / PGIII
Water hazard class (WGK, DE)	3
Hazard codes (EU)	C
Risk statements (R)	10-34
Safety statements (S)	16-26-36/37/39-45

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

25561-30-2

MDL number

MFCD00008269

PubChem substance

24849213

Beilstein registry

2050024

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is N,O-Bis(trimethylsilyl)trifluoroacetamide used for?

It is primarily used as a derivatization reagent in analytical chemistry, particularly for gas chromatography (GC), to increase the volatility and thermal stability of compounds with active hydrogen atoms.

What are the CAS number and formula for N,O-Bis(trimethylsilyl)trifluoroacetamide?

The CAS number is 25561-30-2 and the linear formula is CF3C[=NSi(CH3)3]OSi(CH3)3.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies N,O-Bis(trimethylsilyl)trifluoroacetamide with an assay of ≥99% and is designated for GC derivatization. We do not represent this product as USP, BP, or EP grade.

What are the main safety hazards associated with this chemical?

This compound is flammable (Flash Point: 24 °C) and causes severe skin burns and eye damage. Handle with appropriate personal protective equipment, including gloves, eye protection, and respiratory protection, in a well-ventilated area.

How is N,O-Bis(trimethylsilyl)trifluoroacetamide packed, shipped, and exported?

This product is carefully packaged in compliance with international shipping regulations for hazardous materials. TSS is experienced in exporting chemicals globally to meet your research and industrial needs.

How can I request a sample or quote for N,O-Bis(trimethylsilyl)trifluoroacetamide?

To request a sample or obtain a quote, please visit the product page on the TSS website and utilize the 'Request Sample' or 'Request Quote' functions, or contact our sales team directly.