N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin

CAS 149231-65-2C33H39N5O10Biochemicals & Reagents

N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin (CAS: 149231-65-2) is a fluorogenic peptide substrate with the molecular formula C33H39N5O10 and a molecular weight of 665.69 g/mol. This high-purity compound serves as a valuable tool in biochemical research, particularly for the study of caspases and apoptosis. It is widely used for the in vitro assay and characterisation of protease activity.

IUPAC: Ac-YVAD-AMC
Synonyms: Ac-YVAD-AMC

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N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin (C33H39N5O10) — chemical structure, CAS 149231-65-2; Life Science, fine chemical supplied by Tech Serve Solutions

A-AMApoptosis EnzymesApoptosis and Cell CycleApplication IndexBioactive Small MoleculesBiochemicals and ReagentsCaspase 1Caspase 4Caspase SubstratesCaspases

01 /Applications

Caspase Activity Assays

N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin is a specific substrate for various caspases, enabling researchers to quantify their enzymatic activity in biological samples. This is crucial for understanding cellular processes like apoptosis.

Apoptosis Research

As a key marker for caspase activation, this compound is instrumental in studying the biochemical pathways of programmed cell death. Its use helps elucidate the role of caspases in diverse physiological and pathological conditions.

Protease Characterisation

The substrate is employed to identify and characterise the substrate specificity of proteases, especially cysteine proteases within the caspase family. This aids in the development of targeted inhibitors and therapeutic agents.

Biochemical Research Tool

This fluorogenic substrate provides a sensitive and reliable method for detecting enzymatic hydrolysis, making it a versatile reagent in general biochemical studies involving enzyme kinetics and signalling pathways.

02 /Properties

Molecular weight	665.69
Empirical formula	C33H39N5O10
Assay	≥99% (TLC)
Form	powder
Solubility	DMSO: soluble10 mM (The solution is stable in the dark at −20 °C for more than 1 month if no water is present.)
Storage temperature	−20°C
Composition	Peptide content, ≥80%

03 /Identifiers & registry

CAS number

149231-65-2

MDL number

MFCD00171369

04 /Additional specifications

Gene Information	human ... CASP1(834), CASP4(837)mouse ... CASP1(12362), CASP4(12363)rat ... CASP1(25166), CASP4(114555)

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin used for?

This compound is primarily used as a fluorogenic substrate for the assay and characterisation of caspase enzyme activity, particularly in apoptosis research and the study of protease function.

What is the CAS number and formula for N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin?

The CAS number is 149231-65-2, and its empirical formula is C33H39N5O10.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product with an assay of ≥99% by TLC. We do not represent this product as USP, BP, or EP grade.

What are the safety and handling requirements for this chemical?

This compound has a WGK Germany rating of 3, indicating it is hazardous. It should be handled with appropriate personal protective equipment in a well-ventilated area. Refer to the Safety Data Sheet for detailed information.

How is N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin stored and shipped?

The recommended storage temperature is -20°C. TSS ensures products are packed and exported securely to maintain stability and comply with all relevant shipping regulations.