Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide
Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide (CAS: 95713-52-3) is a chemical compound with the molecular formula C9H9FN4O5 and a molecular weight of 272.19 g/mol. This reagent is primarily utilised for the derivatisation and subsequent High-Performance Liquid Chromatography (HPLC) resolution and quantitation of both D- and L-amino acids. It is an effective tool for peptide analysis and characterisation, offering clear detection capabilities.
- IUPAC
- FDAA,Marfey’s reagent
- Synonyms
- C9H9FN4O5FDAAMarfey’s reagent

Amino Acid Analysis
Utilised for the derivatisation of amino acids, enabling their subsequent resolution and quantitation using HPLC techniques. This allows for precise analysis of amino acid profiles in various samples.
Peptide Characterisation
Serves as a valuable reagent in the characterisation of peptides. By derivatising amino acid residues, it facilitates detailed analysis and identification of peptide structures.
HPLC Derivatisation
This compound is employed as a derivatising agent in HPLC analysis. It reacts with amino acids to form derivatives that exhibit enhanced detectability and separation characteristics.
Biochemical Research
A useful reagent for biochemical applications, particularly in the study of amino acids and peptides. It aids in understanding the composition and function of biological molecules.
| Molecular weight | 272.19 |
|---|---|
| Empirical formula | C9H9FN4O5 |
| Form | powder |
| Storage temperature | 2-8°C |
| Application | For derivatization and resolution of amino acids. Derivatized D- and L-amino acids can be resolved and quantitated by HPLC. |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide used for?
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Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide is used for the derivatisation and subsequent resolution and quantitation of D- and L-amino acids by HPLC. It is also applied in peptide analysis and characterisation.
What are the CAS number and formula for this compound?
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The CAS number is 95713-52-3 and the empirical formula is C9H9FN4O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical as a powder, intended for research and analytical purposes. Specific assay/grade details are available upon request; it is not represented as USP, BP, EP, or pharmacopoeia grade.
How should Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide be handled safely?
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This compound is classified as a warning substance (GHS07) and may cause skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Use with appropriate personal protective equipment including a dust mask (N95 type), eyeshields, and gloves in a well-ventilated area. Avoid breathing dust.
How is this chemical shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. This product can be packed and exported according to international shipping regulations. Please contact us for specific shipping and export details.
How can I request a sample or quote?
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To request a sample or a quotation for Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide, please visit the Tech Serve Solutions website or contact our sales department directly with your inquiry.
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