Leupeptin hydrochloride
Leupeptin hydrochloride (CAS 24125-16-4) is a microbial-derived peptide aldehyde, chemically identified as C20H38N6O4 · HCl with a molecular weight of 463.01 g/mol. This potent protease inhibitor is widely utilised in biochemical research for its ability to block serine, cysteine, and some metalloproteases. It plays a crucial role in preventing protein degradation during sample preparation and in various enzymatic assays, preserving protein integrity in cellular and molecular biology studies.
- IUPAC
- Acetyl-Leu-Leu-Arg-al hydrochloride
Protease Inhibition in Research
Leupeptin hydrochloride is a key reagent in molecular biology and biochemistry laboratories to inhibit the activity of serine and cysteine proteases. This application is critical for preventing protein degradation during cell lysis, protein purification, and the analysis of sensitive protein samples.
Cellular and Biochemical Studies
Its application extends to studying cellular processes where proteolytic activity might interfere with experimental outcomes. By inhibiting proteases, it helps researchers elucidate protein function and signalling pathways with greater accuracy.
Sample Preservation
This compound is frequently included in lysis buffers and storage solutions to preserve the integrity of proteins and peptides. This ensures that downstream analyses, such as Western blotting or mass spectrometry, yield reliable results.
| Molecular weight | 463.01 |
|---|---|
| Empirical formula | C20H38N6O4 · HCl |
| Assay | ≥70% (HPLC) |
| Storage temperature | −20°C |
| Biological source | microbial |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Leupeptin hydrochloride used for?
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Leupeptin hydrochloride is primarily used as a protease inhibitor in biochemical and molecular biology research to prevent protein degradation. It inhibits serine, cysteine, and some metalloproteases, aiding in the preservation of protein integrity during sample preparation and analysis.
What is the CAS number and formula for Leupeptin hydrochloride?
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The CAS number for Leupeptin hydrochloride is 24125-16-4, and its empirical formula is C20H38N6O4 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Leupeptin hydrochloride with an assay of ≥70% by HPLC. This product is intended for research purposes and is not represented as USP, BP, EP, or pharmacopoeia grade.
How should Leupeptin hydrochloride be handled and stored?
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Handle Leupeptin hydrochloride with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It should be stored at -20°C to maintain its stability and efficacy.
Is Leupeptin hydrochloride a controlled substance?
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Based on the provided data, Leupeptin hydrochloride is not listed as a controlled substance. However, users should always comply with local regulations regarding its handling and use.
How is Leupeptin hydrochloride packed and shipped?
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Leupeptin hydrochloride is typically packed in secure containers suitable for chemical reagents and exported globally by Tech Serve Solutions, adhering to international shipping standards for chemical compounds.
How can I request a quote or sample for Leupeptin hydrochloride?
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To request a quote or a sample of Leupeptin hydrochloride, please contact our sales department through the TSS website or by direct enquiry, providing your specific requirements.
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