L-(−)-Dithiothreitol
L-(−)-Dithiothreitol (CAS: 16096-97-2; C4H10O2S2; MW: 154.25 g/mol), also known as Cleland’s Reagent, is a potent reducing agent. It is crucial for protecting sulfhydryl groups from oxidation and for maintaining the reduced state of proteins. Its ability to reduce disulfide bonds makes it invaluable in biochemical research, particularly in protein chemistry and molecular biology applications.
- IUPAC
- (−)-Cleland’s Reagent,(2R,3R)-1,4-Dimercapto-2,3-butanediol,L-DTT,Cleland reagent chiral
- Synonyms
- Cleland’s Reagent(2R,3R)-1,4-Dimercapto-2,3-butanediolL-DTTCleland reagent chiral
Reducing Agent in Biochemistry
L-(−)-Dithiothreitol is widely employed to maintain proteins in their reduced state by cleaving disulfide bonds, essential for protein structure and function studies.
Protecting Sulfhydryl Groups
It serves to protect free sulfhydryl groups from irreversible oxidation during protein purification and storage, preserving enzyme activity.
Enhancing Protein Solubility
By reducing disulfide bonds, DTT can help in solubilising aggregated or denatured proteins, facilitating their analysis and manipulation.
Reagent in Molecular Biology
Used in various molecular biology techniques, including protein electrophoresis and enzyme assays, to ensure optimal reaction conditions.
| Molecular weight | 154.25 |
|---|---|
| Empirical formula | C4H10O2S2 |
| Assay | ≥95% |
| Solubility | H2O: soluble50 mg/mL, clear to hazy, colorless |
| Storage temperature | −20°C |
Hazard statements
- H302Harmful if swallowed
- H312Harmful in contact with skin
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Flash point | 110 °C / 230 °F |
| Transport (UN / ADR) | UN 3335 |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 21/22-36/37/38 |
| Safety statements (S) | 26-36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is L-(−)-Dithiothreitol used for?
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L-(−)-Dithiothreitol (CAS: 16096-97-2) is primarily used as a reducing agent in biochemistry to maintain proteins in their reduced state by cleaving disulfide bonds and to protect sulfhydryl groups from oxidation.
What is the CAS number and formula for L-(−)-Dithiothreitol?
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The CAS number is 16096-97-2 and the empirical formula is C4H10O2S2.
What grade and purity does Tech Serve Solutions supply for L-(−)-Dithiothreitol?
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Tech Serve Solutions supplies L-(−)-Dithiothreitol with an assay of ≥95%. This product is intended for research and laboratory use and is not represented as USP, BP, or EP grade.
How should L-(−)-Dithiothreitol be handled safely?
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L-(−)-Dithiothreitol is harmful if swallowed, in contact with skin, or if inhaled, and causes skin and eye irritation. Handle with appropriate personal protective equipment, including a dust mask (N95), eyeshields, and gloves, in a well-ventilated area. Avoid breathing dust.
Is L-(−)-Dithiothreitol available for export?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including L-(−)-Dithiothreitol.
How can I request a sample or quote for L-(−)-Dithiothreitol?
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Please contact our sales team via the website or by phone to request a sample or a quotation for L-(−)-Dithiothreitol.
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