L-Alanine 4-nitroanilide hydrochloride
L-Alanine 4-nitroanilide hydrochloride (CAS 31796-55-1), with the formula C9H11N3O3 · HCl and a molecular weight of 245.66 g/mol, is a chemical reagent. It is primarily utilised as a chromogenic substrate in biochemical research, particularly for the detection and quantification of enzymatic activity. Its structured format makes it valuable for specific analytical applications in enzyme kinetics and related studies.

Enzyme Activity Assays
L-Alanine 4-nitroanilide hydrochloride serves as a chromogenic substrate for enzymes that cleave the p-nitroanilide bond, releasing p-nitroaniline. This coloured product can be quantified spectrophotometrically, enabling the measurement of enzyme activity.
Biochemical Research
This compound is employed in various biochemical studies to investigate enzyme mechanisms, kinetics, and inhibitor screening. Its well-defined structure facilitates precise experimental design.
Diagnostic Reagent Development
It can be a component in the development of diagnostic assays where specific enzyme activity needs to be detected or measured, contributing to the broader field of biochemical diagnostics.
| Molecular weight | 245.66 |
|---|---|
| Empirical formula | C9H11N3O3 · HCl |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is L-Alanine 4-nitroanilide hydrochloride used for?
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L-Alanine 4-nitroanilide hydrochloride (CAS 31796-55-1) is primarily used as a chromogenic substrate in biochemical research for measuring enzyme activity.
What is the CAS number and chemical formula for L-Alanine 4-nitroanilide hydrochloride?
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The CAS number is 31796-55-1, and the chemical formula is C9H11N3O3 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies L-Alanine 4-nitroanilide hydrochloride for research purposes. The specific assay and purity are detailed in the product's specifications. It is not supplied as USP, BP, EP, or pharmacopoeia grade unless explicitly stated in the specifications.
How should L-Alanine 4-nitroanilide hydrochloride be handled?
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Handling requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or P1 (EN143) respirator filter, due to its potential hazards. It should be stored at -20°C.
Is L-Alanine 4-nitroanilide hydrochloride a controlled substance?
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The provided data does not indicate that L-Alanine 4-nitroanilide hydrochloride is a controlled substance.
How is L-Alanine 4-nitroanilide hydrochloride packed and exported?
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Tech Serve Solutions, a global supplier and exporter since 1998, packs and exports chemicals in compliance with international shipping regulations. Specific packaging details are available upon request.
How can I request a sample or quote for L-Alanine 4-nitroanilide hydrochloride?
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You can request a sample or a quote by contacting our sales team through the details provided on the Tech Serve Solutions website.
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