Iodoacetic acid N-hydroxysuccinimide ester
Iodoacetic acid N-hydroxysuccinimide ester (CAS: 39028-27-8), with the molecular formula C6H6INO4 and a molecular weight of 283.02 g/mol, is a heterobifunctional cross-linking reagent. It offers distinct reactivity towards primary amines and sulfhydryl groups, making it valuable in chemical biology and synthesis. Its primary applications include preparing enzyme immunoconjugates and hapten carrier molecule conjugates through specific amide and thioether linkages.
Amine-Specific Coupling
This reagent readily couples to molecules containing primary amines, forming stable amide bonds under buffered conditions, typically around pH 7.5 (ranging from 6.5-8.5).
Sulfhydryl-Specific Coupling
Following amine coupling, the activated ester can react specifically with free sulfhydryl groups to form a thioether linkage, also at a buffered pH of approximately 7.5 (7.0-8.0).
Bioconjugation
It is instrumental in creating bioconjugates, such as enzyme immunoconjugates and hapten carrier molecule conjugates, by providing a defined two-atom linker for precise molecular assembly.
Protein and Peptide Modification
Used in proteomics and peptide chemistry for modifying proteins and peptides, aiding in structural analysis and the development of conjugates for various research applications.
| Molecular weight | 283.02 |
|---|---|
| Empirical formula | C6H6INO4 |
| Form | powder |
| Solubility | DMF: soluble50 mg/mL |
| Storage temperature | −20°C |
| Application | A heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. Typically, coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5) Second coupling specific for molecules containing free sulfydryl by thioether linkage buffered at pH 7.5 (7.0-8.0). Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates. Provides a 2-atom linker. |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Iodoacetic acid N-hydroxysuccinimide ester used for?
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This heterobifunctional cross-linking reagent is used for coupling molecules containing primary amines and sulfhydryl groups. It is particularly useful for preparing enzyme immunoconjugates and hapten carrier molecule conjugates.
What is the CAS number and molecular formula?
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The CAS number is 39028-27-8, and the molecular formula is C6H6INO4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this compound for research purposes. Purity and specific assay information are available on the product's Certificate of Analysis. We do not represent this product as USP, BP, or EP grade.
What are the main safety concerns when handling this product?
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This product is classified as a Warning with hazards including skin irritation (H315), serious eye irritation (H319), and respiratory tract irritation (H335). Appropriate personal protective equipment, including a dust mask (N95), eyeshields, and gloves, should be worn. Work should be conducted in a well-ventilated area.
How is this chemical packed and shipped?
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Our chemicals are carefully packed to ensure integrity during transit and are exported globally in compliance with all relevant regulations.
How can I request a quote or sample?
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To request a quote or sample, please use the contact form on our website or contact our sales department directly with your specific requirements.
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