Indoxyl β-D-glucoside
Indoxyl β-D-glucoside (CAS 487-60-5), with the molecular formula C14H17NO6 and a molecular weight of 295.29 g/mol, is a biochemical reagent. It is primarily recognised as a chromogenic substrate and is extensively used in enzyme assays, particularly for detecting beta-glucosidase activity. This compound plays a role in biochemical research, molecular biology, and studies involving post-translational modifications and proteomics, where precise enzymatic activity monitoring is crucial.
- IUPAC
- 3-(β-D-Glucosido)indole,Plant indican
- Synonyms
- 3-(β-D-Glucosido)indolePlant indican
Enzyme Substrate for Beta-Glucosidase Detection
Indoxyl β-D-glucoside serves as a sensitive chromogenic substrate for the enzyme beta-glucosidase. Upon enzymatic cleavage, it releases indoxyl, which dimerises and oxidises to form indigo, a coloured product, allowing for quantitative or qualitative assessment of enzyme activity in various biological samples.
Biochemical Research and Assays
It is a valuable tool in biochemical research, enabling researchers to study enzyme kinetics, inhibition, and function. Its use extends to molecular biology and diagnostics for detecting the presence or activity of beta-glucosidase in cellular extracts or other biological matrices.
Glycobiology Studies
Within the field of glycobiology, this compound aids in investigating glycosidic bond hydrolysis and the roles of glycosidases in cellular processes. It is employed in research focusing on carbohydrate metabolism and the structure-function relationships of glycoproteins.
Proteomics and Post-Translational Modifications
Indoxyl β-D-glucoside can be utilised in proteomic studies to assess or identify enzyme activity related to post-translational modifications. Monitoring beta-glucosidase activity provides insights into cellular signalling pathways and protein functional analysis.
| Molecular weight | 295.29 |
|---|---|
| Empirical formula | C14H17NO6 |
| Assay | ≥97% |
| Solubility | H2O: soluble0.1 M, clear to slightly hazy |
| Storage temperature | −20°C |
| Impurities tested | ≤0.0005% Phosphorus (P) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Cation traces | Al: ≤0.005%Ca: ≤0.0005%Cu: ≤0.0005%Fe: ≤0.0005%K: ≤0.005%Mg: ≤0.0005%NH4+: ≤0.05%Na: ≤0.05%Pb: ≤0.001%Zn: ≤0.0005% |
| Anion traces | chloride (Cl-): ≤0.05%sulfate (SO42-): ≤0.05% |
| Ignition residue | <0.1% |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Indoxyl β-D-glucoside used for?
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Indoxyl β-D-glucoside is primarily used as a chromogenic substrate for detecting beta-glucosidase activity in biochemical assays, molecular biology, glycobiology, and proteomic research. It is also employed in studies of post-translational modifications.
What are the CAS number and formula for Indoxyl β-D-glucoside?
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The CAS number for Indoxyl β-D-glucoside is 487-60-5, and its molecular formula is C14H17NO6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Indoxyl β-D-glucoside with an assay of ≥97%. We do not represent this product as USP, BP, EP, or pharmacopoeia grade; it is intended for research and laboratory use.
How should Indoxyl β-D-glucoside be handled and stored?
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Handle Indoxyl β-D-glucoside with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. It should be stored at -20°C. It is classified with WGK Germany 3, indicating a high hazard to water.
Is Indoxyl β-D-glucoside a controlled substance?
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The provided data does not indicate that Indoxyl β-D-glucoside is a controlled substance.
How is Indoxyl β-D-glucoside shipped and exported?
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As a global supplier and exporter, Tech Serve Solutions has extensive experience in shipping and exporting fine chemicals like Indoxyl β-D-glucoside worldwide, adhering to all relevant regulations.
How can I request a quote or sample of Indoxyl β-D-glucoside?
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To request a quote or sample of Indoxyl β-D-glucoside, please contact our sales department through the TSS website or by phone, providing your specific requirements.
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