Hoechst 34580
Hoechst 34580 (CAS: 911004-45-0, Formula: C27H29N7 · 3HCl) is a trihydrochloride salt of a benzimidazole derivative. This fluorescent compound is primarily utilised in biochemical research and diagnostics. Its spectral properties make it valuable for various detection and staining applications, aiding in the visualisation and analysis of biological samples. Supplied by Tech Serve Solutions, it meets stringent purity standards for reliable experimental outcomes.
- IUPAC
- N,N-Dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′-yl]benzenamine trihydrochloride,HOE 34580

Fluorescent Staining
Hoechst 34580 acts as a fluorescent stain, employed in microscopy and imaging techniques. Its excitation and emission spectra allow for the selective visualisation of cellular structures or nucleic acids in biological specimens.
Biochemical Detection
This reagent is valuable for detecting and quantifying specific molecules or processes in biochemical assays. Its fluorescence properties enable sensitive detection methods in research settings.
Luminescent Probes
As a luminescent compound, Hoechst 34580 can be utilised as a probe in various analytical procedures. Its distinct spectral signature facilitates its use in specialised detection systems.
| Empirical formula | C27H29N7 · 3HCl |
|---|---|
| Assay | ≥98.0% (HPLC) |
| Solubility | H2O: soluble1 mg/mL, clear, slightly yellow to deep greenish-yellow |
| Storage temperature | −20°C |
| Fluorescence | λex 357 nm; λem 490 nm±10 nm in H2O (free dye) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Hoechst 34580 used for?
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Hoechst 34580 is primarily used as a fluorescent stain in biochemical research and diagnostics, and for luminescent detection applications.
What are the CAS number and chemical formula for Hoechst 34580?
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The CAS number for Hoechst 34580 is 911004-45-0 and its chemical formula is C27H29N7 · 3HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Hoechst 34580 with an assay of ≥98.0% (HPLC). This is a research or technical grade product and TSS does not represent it as USP, BP, or EP grade.
How should Hoechst 34580 be stored and handled?
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Hoechst 34580 should be stored at -20°C. It is classified under WGK Germany 3, indicating a high hazard to water. Handle with appropriate personal protective equipment in a well-ventilated area.
Is Hoechst 34580 available for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including Hoechst 34580. Please contact us for export details.
How can I request a sample or quote for Hoechst 34580?
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You can request a sample or a quote for Hoechst 34580 by contacting our sales team through the TSS website or by direct enquiry.
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