Glutaryl-L-phenylalanine 7-amido-4-methylcoumarin
Glutaryl-L-phenylalanine 7-amido-4-methylcoumarin (CAS: 58632-47-6) is a key fluorescent enzyme substrate with the molecular formula C24H24N2O6 and a molecular weight of 436.46 g/mol. This compound serves as a sensitive reagent in biochemical research, primarily utilized for the detection and quantification of specific enzymatic activities. Its fluorescence properties make it invaluable for enzyme assays and related diagnostic applications.
- IUPAC
- Glut-Phe-AMC,N-Glutaryl-L-phenylalanine-4-methyl-7-coumarinylamide
- Synonyms
- Glut-Phe-AMCN-Glutaryl-L-phenylalanine-4-methyl-7-coumarinylamide
Enzyme Activity Detection
Used as a specific fluorogenic substrate to detect and measure the activity of certain proteases, particularly those that cleave the glutaryl-phenylalanine bond. The release of the 4-methylcoumarin moiety upon enzymatic hydrolysis generates fluorescence, allowing for sensitive detection.
Biochemical Research
Serves as a valuable tool in molecular biology and biochemistry laboratories for studying enzyme kinetics, characterizing enzyme mechanisms, and screening for enzyme inhibitors. Its predictable hydrolysis allows for quantitative analysis of enzyme performance.
Fluorescent Probes
Acts as a fluorescent probe in various biological assays. The excitation and emission wavelengths (e.g., λex 325 nm; λem 395 nm in H2O) are characteristic and enable its use in fluorescence spectroscopy for monitoring enzymatic reactions.
| Molecular weight | 436.46 |
|---|---|
| Empirical formula | C24H24N2O6 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 325 nm; λem 395 nm in H2Oλex 380 nm; λem 460 nm (Reaction product) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Glutaryl-L-phenylalanine 7-amido-4-methylcoumarin used for?
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This compound is primarily used as a fluorogenic substrate for detecting and quantifying the activity of specific enzymes in biochemical and research applications.
What are the CAS number and molecular formula?
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The CAS number is 58632-47-6 and the molecular formula is C24H24N2O6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research and laboratory use. The specific grade and purity are detailed in the product specifications. TSS does not represent this product as USP, BP, EP, or other pharmacopoeial grades.
What are the safety and handling recommendations?
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Refer to the safety data sheet for comprehensive handling information. Recommended personal protective equipment includes eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The substance has a WGK Germany classification of 3, indicating it is hazardous to water.
How is this chemical typically stored and shipped?
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The recommended storage temperature is -20°C. Tech Serve Solutions handles the packaging, shipping, and export of chemicals in compliance with all relevant regulations.
How can I request a sample or quote?
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Please contact our sales department through the website or by phone to request a sample or obtain a quotation for Glutaryl-L-phenylalanine 7-amido-4-methylcoumarin.
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