Fura 2-AM
Fura 2-AM (CAS 108964-32-5), with the empirical formula C44H47N3O24 and a molecular weight of 1001.85 g/mol, is a cell-permeable fluorescent probe meticulously designed for the quantitative determination of cytosolic Ca2+ levels. Following intracellular metabolism to its active form, Fura 2, it serves as a critical reagent in cell biology and biochemical research for investigating calcium signaling pathways.
- IUPAC
- Fura-2 pentakis(acetoxymethyl) ester
- Synonyms
- Fura-2 pentakis(acetoxymethyl) ester
Calcium Ion Measurement
Fura 2-AM is employed as a cell-permeable fluorescent probe to accurately measure cytosolic free calcium (Ca2+) concentrations. Its ability to be metabolized into the active Fura 2 ligand within cells makes it invaluable for dynamic Ca2+ studies.
Cell Signaling Research
This compound is instrumental in studying cellular signaling cascades that are regulated by calcium ions. Researchers utilize Fura 2-AM to elucidate the complex roles of calcium in various cellular processes, including neurotransmission and muscle contraction.
Fluorescence Microscopy and Flow Cytometry
As a fluorescent indicator, Fura 2-AM is suitable for use in fluorescence microscopy and flow cytometry, enabling visualization and quantification of intracellular calcium changes in individual cells or cell populations.
| Molecular weight | 1001.85 |
|---|---|
| Empirical formula | C44H47N3O24 |
| Assay | ≥95.0% (HPLC) |
| Form | powder |
| Solubility | DMSO: soluble |
| Storage temperature | −20°C |
| Application | Cell permeable fluorescent probe for Ca2+ used to determine cystolic Ca2+; metabolized in vivo to the active ligand Fura 2. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 355 nm; λem 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca2+ |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fura 2-AM primarily used for?
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Fura 2-AM is a cell-permeable fluorescent probe used to determine cytosolic Ca2+ levels. It is metabolized in vivo to the active ligand Fura 2.
What is the CAS number and molecular formula for Fura 2-AM?
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The CAS number for Fura 2-AM is 108964-32-5, and its empirical formula is C44H47N3O24.
What grade and purity does Tech Serve Solutions supply for Fura 2-AM?
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Tech Serve Solutions supplies Fura 2-AM with an assay of ≥95.0% (HPLC). This product is intended for research purposes and is not represented as USP, BP, EP, or other pharmacopoeia grades.
What are the safety and handling recommendations for Fura 2-AM?
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When handling Fura 2-AM, it is essential to use personal protective equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used. The compound is classified under WGK Germany 3, indicating a hazard to water.
How is Fura 2-AM shipped and exported by Tech Serve Solutions?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including Fura 2-AM, ensuring compliant packaging and shipping procedures for international distribution.
How can I request a quote or sample for Fura 2-AM?
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To request a quote or a sample of Fura 2-AM, please contact our sales team through the designated channels on the Tech Serve Solutions website.
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