Fast Green FCF
Fast Green FCF (CAS: 2353-45-9) is a synthetic colorant with the molecular formula C37H34N2O10S3Na2 and a molecular weight of 808.85 g/mol. This dye is predominantly utilized for protein staining in various electrophoresis techniques, including SDS-PAGE and isoelectric focusing. Its linear staining range surpasses that of Brilliant Blue R, offering enhanced sensitivity for protein detection after electrophoretic separation. Tech Serve Solutions supplies this reagent for laboratory and research applications.
- IUPAC
- Food green 3

Protein Staining in Electrophoresis
Fast Green FCF is widely employed for staining proteins separated by SDS-PAGE, native PAGE, and isoelectric focusing (IEF). Its sensitivity is comparable to or greater than other common protein stains, especially after gel fixation.
Research and Laboratory Reagent
As a specialized laboratory reagent, Fast Green FCF is suitable for biochemical and molecular biology research. It is used in studies involving protein analysis and quantification within gels.
Histology and Cell Biology
While primarily used for protein electrophoresis, Fast Green FCF can also find applications in histology and cell biology as a differential stain, though its primary use remains protein detection.
| Molecular weight | 808.85 |
|---|---|
| Linear formula | C37H34N2O10S3Na2 |
| Application | Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R. |
| Composition | Dye content, ≥85% |
| Melting point | 290 °C (dec.)(lit.) |

Hazard statements
- H341Suspected of causing genetic defects
Precautionary statements
- P281Use personal protective equipment as required
| Protective equipment | Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 2 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 68 |
| Safety statements (S) | 36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 42053 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fast Green FCF used for?
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Fast Green FCF (CAS: 2353-45-9) is primarily used for protein staining in electrophoresis techniques such as SDS-PAGE and isoelectric focusing (IEF). It offers linear staining over a wide range of protein concentrations and is effective for detecting proteins after gel fixation.
What are the CAS number and formula for Fast Green FCF?
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The CAS number for Fast Green FCF is 2353-45-9, and its molecular formula is C37H34N2O10S3Na2.
What grade and purity does Tech Serve Solutions supply for Fast Green FCF?
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Tech Serve Solutions supplies Fast Green FCF with a dye content of ≥85%. This product is intended for research and laboratory use. Tech Serve Solutions does not represent this product as USP, BP, or EP grade.
What are the safety and handling precautions for Fast Green FCF?
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Fast Green FCF is classified with the GHS symbol GHS08 and signal word Warning. It carries the hazard statement H341 (Suspected of causing genetic defects). Recommended personal protective equipment includes eyeshields and gloves. Ensure adequate ventilation and avoid exposure.
How is Fast Green FCF packed and exported by TSS?
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Tech Serve Solutions carefully packs and exports Fast Green FCF, ensuring compliance with international shipping regulations for chemical reagents. Specific packaging details depend on order quantity and destination.
How can I request a quote or sample for Fast Green FCF?
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To request a quote or a sample of Fast Green FCF, please contact our sales team through the website or provide your requirements via email. Our specialists will assist you promptly.
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