DOTAP methosulfate
DOTAP methosulfate (CAS: 144189-73-1), with the molecular formula C43H83NO8S, is a cationic liposome-forming compound. It is specifically designed for the efficient transfection of negatively charged molecules, such as DNA and RNA, into eukaryotic cells. This reagent facilitates the fusion of liposomes with cell membranes, leading to the release of genetic material into the cytoplasm with minimal cytotoxic effects. It is supplied as a clear to hazy, colourless to faint yellow liquid.
- IUPAC
- N-(2,3-Dioleoyloxy-1-propyl)trimethylammonium methyl sulfate,Transfection reagent
DNA and RNA Transfection
DOTAP methosulfate is an effective cationic liposome-forming compound for transfecting DNA, RNA, and other negatively charged molecules into eukaryotic cells. It forms stable complexes with nucleic acids, promoting efficient delivery into the cytoplasm.
Lipid-based Transfection Reagent
As a key component in cationic liposomes, DOTAP facilitates membrane fusion, enabling the release of loaded molecules into the cell. This method is notable for achieving efficient transfection without significant cytotoxic effects.
Molecular Biology Research
This reagent is suitable for molecular biology applications, particularly in studies involving gene expression, signal transduction, and cell signaling, where the introduction of genetic material is required.
| Molecular weight | 774.19 |
|---|---|
| Empirical formula | C43H83NO8S |
| Grade | for molecular biology |
| Form | liquid (MES buffered saline) |
| Colour | clear to hazy colorless to faint yellow |
| Concentration | 1 mg/mL |
| Storage temperature | 2-8°C |
| Application | Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded. |
| Protective equipment | Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Usage | sufficient for 40 transfections (10 cm dishes) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is DOTAP methosulfate used for?
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DOTAP methosulfate is a cationic liposome-forming compound used for the efficient transfection of DNA, RNA, and other negatively charged molecules into eukaryotic cells. It aids in the delivery of these molecules into the cytoplasm with minimal cytotoxicity.
What are the CAS number and formula for DOTAP methosulfate?
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The CAS number for DOTAP methosulfate is 144189-73-1, and its molecular formula is C43H83NO8S.
What grade and purity does Tech Serve Solutions supply for DOTAP methosulfate?
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Tech Serve Solutions supplies DOTAP methosulfate in a grade suitable for molecular biology applications. We do not represent this product as meeting USP, EP, or BP pharmacopoeial standards.
How should DOTAP methosulfate be handled safely?
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When handling DOTAP methosulfate, it is essential to wear eyeshields and gloves. This compound is classified under WGK Germany 3, indicating a potential hazard to water. Ensure appropriate safety measures are in place during use and disposal.
How is DOTAP methosulfate shipped and exported?
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DOTAP methosulfate is typically shipped and exported under refrigeration to maintain its stability and efficacy. Please contact us for specific shipping and export details relevant to your location.
How can I request a sample or quote for DOTAP methosulfate?
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To request a sample or a quote for DOTAP methosulfate, please contact our sales team through the website or by phone. We will be pleased to assist you with your inquiry.
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