Disperse Orange 13
Disperse Orange 13 (CAS 6253-10-7) is an azo dye with the molecular formula C22H18N4O2 and a molecular weight of 352.39 g/mol. It presents as an orange to brown powder. This compound is primarily utilised as a staining agent in various laboratory applications, particularly within cell biology and histology, for differentiating cellular structures. Its distinct colour and chemical properties make it valuable for microscopic examination and research purposes.
- IUPAC
- 4-[4-(Phenylazo)-1-naphthylazo]phenol

Histological Staining
Disperse Orange 13 serves as a stain in histology for visualising cellular components. Its application aids researchers in differentiating tissue structures under microscopic examination, facilitating diagnostic and research studies.
Cell Biology Research
In cell biology, this dye is employed for staining purposes to enhance the visibility of cellular elements. It is useful in various research protocols requiring precise visualisation of cellular morphology and organisation.
Dye for Microscopy
As a specialist dye, Disperse Orange 13 is utilised in microscopy to provide contrast and detail in biological samples. Its absorption maximum at 427 nm contributes to its utility in specific staining procedures.
| Molecular weight | 352.39 |
|---|---|
| Linear formula | C6H5N=NC10H6N=NC6H4OH |
| Melting point | 149-153 °C(lit.) |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-37/39 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 26080 |
|---|---|
| Absorption | λmax 427 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Disperse Orange 13 used for?
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Disperse Orange 13 is primarily used as a staining agent in histology and cell biology research for microscopic examination. It helps visualise and differentiate cellular structures and tissues.
What is the CAS number and chemical formula for Disperse Orange 13?
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The CAS number for Disperse Orange 13 is 6253-10-7, and its chemical formula is C22H18N4O2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Disperse Orange 13 in a grade suitable for laboratory research. The specific assay and purity are detailed in the product specifications. We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should Disperse Orange 13 be handled safely?
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Disperse Orange 13 is classified as a warning substance, causing skin irritation (H315), eye irritation (H319), and respiratory irritation (H335). Recommended personal protective equipment includes a dust mask type N95 (US), eyeshields, and gloves. Handle in a well-ventilated area and avoid breathing dust.
Is Disperse Orange 13 packed and shipped internationally?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including Disperse Orange 13, and can arrange for international shipping according to regulations.
How can I request a quote or sample for Disperse Orange 13?
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To request a quote or a sample of Disperse Orange 13, please contact our sales team via the website or direct inquiry, providing your specific requirements.
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