Direct Blue 15
Direct Blue 15 (CAS: 2429-74-5), a tetrasodium salt with the formula C34H24N6O16S4Na4 and a molecular weight of 992.80 g/mol, is a vital stain used in various biological and histological applications. It serves as a potential substitute for trypan blue in certain staining procedures and is specifically noted for its suitability in viability studies of collagenase-treated rat liver cells and within the Histopaque® system.
Biological Staining
Direct Blue 15 is employed as a stain in various biological contexts. It can function as a substitute for trypan blue in specific staining applications, aiding in cellular visualisation and differentiation.
Histology
In histological preparations, Direct Blue 15 is utilized for staining tissues. Its application extends to assisting with cellular characterisation and the examination of tissue morphology.
Viability Studies
This dye is suitable for viability studies, particularly with collagenase-treated rat liver cells. It helps assess cell membrane integrity and differentiate between viable and non-viable cells.
Histopaque® System
Direct Blue 15 is compatible with the Histopaque® system, a density gradient medium used for cell separation and analysis. Its inclusion supports specific research protocols within this system.
| Molecular weight | 992.80 |
|---|---|
| Linear formula | C34H24N6O16S4Na4 |
| Application | May be used as a substitute for trypan blue for some biological and staining applications. |
| Suitability | suitable for Histopaque® systemsuitable for viability studies of collagenase-treated rat liver cells |
Hazard statements
- H350May cause cancer
Precautionary statements
- P201Obtain special instructions before use
- P308IF exposed or concerned
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | T |
| Risk statements (R) | 45 |
| Safety statements (S) | 53-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 24400 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Direct Blue 15 used for?
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Direct Blue 15 is used in biological staining, histology, viability studies of collagenase-treated rat liver cells, and within the Histopaque® system. It can also be used as a substitute for trypan blue in some staining applications.
What is the CAS number and chemical formula for Direct Blue 15?
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The CAS number for Direct Blue 15 is 2429-74-5, and its linear chemical formula is C34H24N6O16S4Na4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Direct Blue 15 with a specified molecular weight of 992.80 g/mol and Colour Index Number 24400. The specific assay/grade is not detailed beyond its suitability for the stated applications. TSS does not represent this product as USP, BP, EP, or other pharmacopoeial grades.
What are the safety and handling requirements for Direct Blue 15?
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Direct Blue 15 is classified with GHS08 symbol and 'Danger' signal word, carrying hazard statement H350 (May cause cancer). Precautionary statements include P201 (Obtain special instructions before use) and P308 + P313 (If exposed or concerned: Get medical advice/attention). Handle with extreme care in a well-ventilated area and avoid exposure.
How is Direct Blue 15 packed and shipped?
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Direct Blue 15 is carefully packed to ensure stability and integrity during transit and is available for global export by Tech Serve Solutions.
How can I request a sample or quote for Direct Blue 15?
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To request a sample or obtain a quote for Direct Blue 15, please contact the Tech Serve Solutions sales department through our website or by phone.
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