Dimidium bromide
Dimidium bromide (CAS: 518-67-2; Formula: C20H18BrN3) is a fluorescent intercalating agent with a molecular weight of 380.28 g/mol. It is primarily employed as a sensitive probe for nucleic acids, exhibiting fluorescence upon binding. This compound is a valuable tool in biochemical research and molecular biology for staining and detecting DNA and RNA.
- IUPAC
- 3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide,Trypadine
- Synonyms
- 3,8-Diamino-5-methyl-6-phenylphenanthridinium bromideTrypadine

Nucleic Acid Staining
Dimidium bromide serves as an intercalating probe for nucleic acids. Its fluorescent properties enable the visualisation and detection of DNA and RNA in various biological samples and experimental settings.
Biochemical Research
Utilised as a fluorescent stain in biochemical applications, Dimidium bromide assists in the study of nucleic acid structure and interactions. It is commonly used in electrophoresis and other molecular biology techniques.
Fluorescent Probes
As a fluorescent probe, Dimidium bromide allows for the sensitive detection of genetic material. Its ability to intercalate into the double helix of nucleic acids makes it a useful reagent for analytical purposes.
| Molecular weight | 380.28 |
|---|---|
| Empirical formula | C20H18BrN3 |
| Assay | ≥95% |
| Storage temperature | 2-8°C |
| Application | Intercalating probe for nucleic acids.1 |
| Melting point | 243-248 °C(lit.) |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Dimidium bromide used for?
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Dimidium bromide is primarily used as an intercalating probe for nucleic acids, functioning as a fluorescent stain in biochemical research and molecular biology.
What is the CAS number and chemical formula for Dimidium bromide?
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The CAS number for Dimidium bromide is 518-67-2, and its chemical formula is C20H18BrN3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Dimidium bromide with an assay of ≥95%. This product is intended for research and laboratory use; TSS does not represent it as USP, BP, or EP grade.
How should Dimidium bromide be handled and stored?
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Dimidium bromide is classified as a Warning substance, with hazards including skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Handle with appropriate personal protective equipment: a dust mask type N95 (US), eyeshields, and gloves. Store at 2-8°C.
How is Dimidium bromide packed, shipped, and exported?
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Dimidium bromide is carefully packed to ensure safe transit and is available for global export by Tech Serve Solutions, a reputable supplier since 1998.
How can I request a sample or quote for Dimidium bromide?
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To request a sample or a quotation for Dimidium bromide, please contact our sales team via the website or by phone.
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