Diethyl pyrocarbonate
Diethyl pyrocarbonate (CAS: 1609-47-8) is an organic chemical compound with the molecular formula O(COOC2H5)2 and a molecular weight of 162.14 g/mol. This versatile reagent is primarily employed in biochemistry for the modification of histidine and tyrosine residues in proteins. It also serves as a robust probe for detecting structural disruptions in double-stranded DNA by reacting with unstacked bases.
- IUPAC
- DEP,DEPC,Diethyl dicarbonate,Diethyl oxydiformate,Ethoxyformic acid anhydride
- Synonyms
- DEPDEPCDiethyl dicarbonateDiethyl oxydiformateEthoxyformic acid anhydride
Protein Modification
Diethyl pyrocarbonate selectively modifies histidine and tyrosine residues within protein structures. This application is crucial for biochemical studies investigating protein function and structure.
DNA Structure Analysis
It acts as a reliable probe for identifying structural disruptions in double-stranded DNA. The compound reacts with fully or partially unstacked DNA bases, providing insights into DNA conformation.
Chemical Synthesis
As an organic building block, diethyl pyrocarbonate is utilized in various chemical synthesis pathways. Its reactivity makes it a valuable intermediate in the production of more complex organic molecules.
| Molecular weight | 162.14 |
|---|---|
| Linear formula | O(COOC2H5)2 |
| Assay | 99% (NT) |
| Boiling point | 93-94 °C/18 mmHg(lit.) |
| Density | 1.121 g/mL at 20 °C1.101 g/mL at 25 °C(lit.) |
| Refractive index | n20/D 1.398(lit.)n20/D 1.398 |
| Storage temperature | 2-8°C |
| Impurities tested | ≤10 ppm aromatic substances |
| Application | Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.1 |
Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter |
|---|---|
| Flash point | 69 °C / 156.2 °F |
| Transport (UN / ADR) | UN 1993 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Diethyl pyrocarbonate used for?
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Diethyl pyrocarbonate is used for the modification of histidine and tyrosine residues in proteins and as a probe for structural disruptions in dsDNA. It also serves as a building block in chemical synthesis.
What is the CAS number and formula for Diethyl pyrocarbonate?
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The CAS number for Diethyl pyrocarbonate is 1609-47-8, and its linear formula is O(COOC2H5)2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Diethyl pyrocarbonate with an assay of 99% (NT). We do not represent this product as USP, BP, or EP grade.
How should Diethyl pyrocarbonate be handled safely?
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Diethyl pyrocarbonate is harmful if swallowed, causes skin irritation, causes serious eye irritation, and may cause respiratory irritation. Always use appropriate personal protective equipment, including eyeshields, gloves, and a suitable respirator, and ensure adequate ventilation.
How is Diethyl pyrocarbonate packed and shipped?
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Diethyl pyrocarbonate is packed and shipped in compliance with UN 1993 / PGIII regulations. Storage is recommended at 2-8°C.
How can I request a quote or sample for Diethyl pyrocarbonate?
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To request a quote or sample of Diethyl pyrocarbonate, please contact the Tech Serve Solutions sales team through our website or by phone.
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