DAF-FM DA
DAF-FM DA (CAS 254109-22-3) is a difluoro-substituted fluorescein derivative with the molecular formula C25H18F2N2O7 and a molecular weight of 496.42. This compound exhibits fluorescence with excitation at 500 nm and emission at 515 nm in DMSO. It is primarily utilised in cell biology research, particularly in studies involving cell signalling, neuroscience, and the detection and metabolism of nitric oxide, as well as in investigating cell stress.
- IUPAC
- 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one,Diaminofluorescein-FM diacetate
- Synonyms
- 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one,Diaminofluorescein-FM diacetate
Cell Biology Research
DAF-FM DA serves as a valuable tool in cell biology for visualizing cellular processes. Its fluorescent properties make it suitable for microscopy and flow cytometry applications.
Nitric Oxide Detection
This compound is employed in the detection and measurement of nitric oxide (NO) in biological systems. It allows researchers to study NO production and its role in cellular functions.
Cell Signaling Studies
DAF-FM DA is used to investigate cell signalling pathways, particularly those influenced by nitric oxide. It aids in understanding the complex communication networks within cells.
Neuroscience Applications
In neuroscience research, this reagent can be used to study NO signalling in neuronal cells, contributing to the understanding of neurological processes and disorders.
| Molecular weight | 496.42 |
|---|---|
| Empirical formula | C25H18F2N2O7 |
| Assay | ≥98% (HPLC) |
| Storage temperature | −20°C |
| Storage Condition | desiccated |
|---|---|
| Fluorescence | λex 500 nm; λem 515 nm in DMSO |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is DAF-FM DA used for?
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DAF-FM DA is primarily used in cell biology research for applications including cell signalling, neuroscience, and the detection and metabolism of nitric oxide, as well as in studying cell stress.
What are the CAS number and formula for DAF-FM DA?
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The CAS number for DAF-FM DA is 254109-22-3, and its empirical formula is C25H18F2N2O7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies DAF-FM DA with a purity of ≥98% (HPLC). This product is intended for research use and is not represented as USP, BP, or EP grade.
How should DAF-FM DA be stored and handled?
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DAF-FM DA should be stored desiccated at -20°C. It is classified under WGK Germany 3, indicating a significant hazard to water.
How is DAF-FM DA packed and shipped?
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DAF-FM DA is carefully packed and exported by Tech Serve Solutions, adhering to all necessary regulations for the safe transport of chemicals.
How can I request a sample or quote for DAF-FM DA?
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To request a sample or quote for DAF-FM DA, please contact our sales team through the website or by phone.
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