DAF-FM
DAF-FM (CAS: 254109-20-1; C21H14F2N2O5) is a difluorinated derivative of diaminofluorescein, primarily utilised as a sensitive fluorescent probe. This compound is instrumental in the study of nitric oxide (NO) production and its related cellular processes. Its high fluorescence quantum yield and photostability make it a valuable tool in cell biology, particularly for visualising NO dynamics in living cells and tissues.
- IUPAC
- 4-Amino-5-methylamino-2′,7′-difluorescein,Diaminofluorescein-FM
- Synonyms
- 4-Amino-5-methylamino-2′,7′-difluorescein,Diaminofluorescein-FM
Nitric Oxide Detection
DAF-FM is widely employed to detect and quantify nitric oxide (NO) due to its selective fluorescent response to NO. It allows for real-time monitoring of NO generation in various biological systems.
Cell Biology Research
Its utility extends to fundamental cell biology studies, enabling researchers to investigate the role of NO in cellular signalling pathways, apoptosis, and stress responses.
Neuroscience Studies
In neuroscience, DAF-FM aids in understanding NO's function as a neurotransmitter or neuromodulator, helping to visualise NO release in neuronal networks.
Nitric Oxide Metabolism
The probe is crucial for investigating the metabolic pathways involving nitric oxide, including its synthesis, diffusion, and degradation within cells and tissues.
| Empirical formula | C21H14F2N2O5 |
|---|---|
| Assay | ≥98% (HPLC) |
| Solubility | DMSO: >1 mg/mL |
| Storage temperature | −20°C |
Hazard statements
- H302Harmful if swallowed
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Storage Condition | desiccated |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is DAF-FM used for?
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DAF-FM (CAS: 254109-20-1) is primarily used as a fluorescent probe for the detection and quantification of nitric oxide (NO) in biological research, including cell biology, neuroscience, and studies on NO metabolism and cell stress.
What are the CAS number and formula for DAF-FM?
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The CAS number for DAF-FM is 254109-20-1, and its empirical formula is C21H14F2N2O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies DAF-FM with an assay of ≥98% (HPLC). We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should DAF-FM be handled and stored?
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DAF-FM is classified as harmful if swallowed (H302). It should be handled with appropriate personal protective equipment and stored desiccated at -20°C.
How is DAF-FM shipped and exported?
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DAF-FM is carefully packaged and exported by Tech Serve Solutions in compliance with all relevant shipping regulations for chemical compounds.
How can I request a sample or quote for DAF-FM?
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To request a sample or a quotation for DAF-FM, please contact the Tech Serve Solutions sales department via our website or direct email.
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