D-Luciferin sodium salt
D-Luciferin sodium salt (CAS: 103404-75-7), a synthetic compound with the molecular formula C11H7N2NaO3S2 and a molecular weight of 302.30 g/mol, serves as a crucial substrate for firefly luciferase. It exhibits a Km of approximately 2 μM, making it highly effective in enzymatic assays. This chemiluminescent compound is primarily utilised in biochemical research and diagnostic applications requiring light emission upon enzymatic reaction.
- IUPAC
- (S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid sodium salt,4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt,Firefly luciferin sodium salt
- Synonyms
- 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium saltFirefly luciferin sodium salt(S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid sodium salt

Luciferase Enzyme Assays
D-Luciferin sodium salt is the essential substrate for the enzyme luciferase, commonly derived from fireflies. Upon reaction with ATP and oxygen in the presence of luciferase, it produces light, making it invaluable for quantitative biological assays.
Chemiluminescent Detection
This compound enables highly sensitive detection methods due to its chemiluminescent properties. It is employed in various research settings for gene expression studies, cell viability assays, and ATP measurements.
Biochemical Research Reagent
As a core bioreagent, D-Luciferin sodium salt is fundamental for investigating enzyme kinetics and mechanisms involving luciferase, supporting fundamental scientific discovery.
| Molecular weight | 302.30 |
|---|---|
| Empirical formula | C11H7N2NaO3S2 |
| Form | lyophilized powder |
| Solubility | H2O: soluble |
| Storage temperature | −20°C |
| Application | Substrate for firefly luciferase with a Km of approx 2 μM. |
| Biological source | synthetic |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 328 nm; λem 532 nm in H2O |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is D-Luciferin sodium salt used for?
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D-Luciferin sodium salt is primarily used as a substrate for firefly luciferase in chemiluminescent assays, enabling detection and quantification in various biochemical and biological research applications.
What is the CAS number and formula for D-Luciferin sodium salt?
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The CAS number for D-Luciferin sodium salt is 103404-75-7, and its empirical formula is C11H7N2NaO3S2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies D-Luciferin sodium salt as a lyophilized powder. While specific assay details may vary, it is supplied for research and technical use and is not represented as USP, BP, or EP grade.
How should D-Luciferin sodium salt be handled?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The substance has a WGK (Germany) rating of 3, indicating it is hazardous to water.
How is D-Luciferin sodium salt shipped and exported?
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D-Luciferin sodium salt is typically stored at -20°C and shipped according to standard chemical handling protocols. Tech Serve Solutions is an experienced global supplier and exporter of fine chemicals.
How can I request a sample or quote for D-Luciferin sodium salt?
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To request a sample or obtain a quote for D-Luciferin sodium salt, please contact our sales team through the website or by phone.
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