Cholesterol 5β,6β-epoxide
Cholesterol 5β,6β-epoxide (CAS 4025-59-6), with the molecular formula C27H46O2, is a significant cholesterol derivative and sterol lipid. This compound is primarily utilised in biochemical research to investigate the biological effects of cholesterol oxidation products. It serves as a valuable reagent in cell culture studies, particularly in understanding cellular responses and metabolic pathways in various cell lines.
- IUPAC
- 5β,6β-Epoxycholestan-3β-ol
- Synonyms
- 5β,6β-Epoxycholestan-3β-olC27H46O2

Cholesterol Oxidation Studies
Cholesterol 5β,6β-epoxide is employed in scientific research to explore the physiological consequences of cholesterol oxidation within biological systems. Its use aids in elucidating the roles of oxysterols in cellular processes.
Cell Culture Applications
This compound is applied to specific cell lines, such as PC12, HepG2, and Caco-2 cells, to observe and analyse the impact of cholesterol oxidation products on cellular behaviour and function.
Biochemical Research Reagent
As a specialised biochemical, Cholesterol 5β,6β-epoxide functions as a research tool for investigating lipid metabolism and sterol pathways in vitro.
| Molecular weight | 402.65 |
|---|---|
| Empirical formula | C27H46O2 |
| Assay | ≥98% |
| Application | Cholesterol 5β,6β-epoxide was used to treat PC12 cells or HepG2 and Caco-2 cells1 to study the effects of cholesterol oxidation.2 |
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/21/22-68/20/21/22 |
| Safety statements (S) | 36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Cholesterol 5β,6β-epoxide used for?
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Cholesterol 5β,6β-epoxide is used in biochemical research to study the effects of cholesterol oxidation products on cell lines like PC12, HepG2, and Caco-2.
What are the CAS number and chemical formula for Cholesterol 5β,6β-epoxide?
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The CAS number for Cholesterol 5β,6β-epoxide is 4025-59-6, and its molecular formula is C27H46O2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Cholesterol 5β,6β-epoxide with an assay of ≥98%. This product is intended for research and laboratory use and is not supplied as USP, BP, EP, or pharmaceutical grade.
How should Cholesterol 5β,6β-epoxide be handled safely?
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When handling Cholesterol 5β,6β-epoxide, it is essential to use appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, faceshields, and gloves. This compound is classified with hazard codes Xn and carries risk statements related to inhalation, skin contact, and ingestion.
How is Cholesterol 5β,6β-epoxide packed, shipped, and exported?
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Tech Serve Solutions packs, ships, and exports Cholesterol 5β,6β-epoxide according to international regulations for chemical transport to ensure safe delivery to researchers and laboratories worldwide.
How can I request a sample or quote for Cholesterol 5β,6β-epoxide?
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To request a sample or a quote for Cholesterol 5β,6β-epoxide, please contact our sales department through the website or by phone.
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