Cholesta-3,5-diene
Cholesta-3,5-diene (CAS 747-90-0), a sterol lipid with the molecular formula C27H44, is a valuable biochemical reagent. This compound serves as a crucial standard in High-Performance Liquid Chromatography (HPLC) for the quality analysis of various edible fats and olive oils. Its well-defined structure and purity make it suitable for research and analytical applications in lipid and cholesterol studies.
- Synonyms
- C27H44

HPLC Standard for Olive Oil Analysis
Cholesta-3,5-diene is employed as a reference standard in HPLC methods to ensure accurate quality control and analysis of different types of olive oils.
Edible Fat Quality Control
This compound is utilised as a standard in analytical procedures for assessing the quality and composition of various edible fats through chromatographic techniques.
Lipid Research and Biochemical Studies
As a derivative of cholesterol, Cholesta-3,5-diene is useful in biochemical research, particularly in studies involving sterol lipids and their metabolic pathways.
| Molecular weight | 368.64 |
|---|---|
| Empirical formula | C27H44 |
| Assay | ≥93% (HPLC) |
| Storage temperature | −20°C |
| Application | Cholesta-3,5-diene was used as standard in HPLC in quality analysis of different types of olive oils1 and edible fats.2 |
| Melting point | 78-80 °C(lit.) |

Hazard statements
- H302Harmful if swallowed
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22 |
| Safety statements (S) | 36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Cholesta-3,5-diene used for?
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Cholesta-3,5-diene is primarily used as a standard in HPLC for the quality analysis of olive oils and edible fats, and in biochemical research concerning sterol lipids.
What is the CAS number and chemical formula for Cholesta-3,5-diene?
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The CAS number is 747-90-0, and its empirical formula (Hill notation) is C27H44.
What grade and purity does Tech Serve Solutions supply for Cholesta-3,5-diene?
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Tech Serve Solutions supplies Cholesta-3,5-diene with an assay of ≥93% by HPLC. We do not represent this product as meeting USP, BP, or EP pharmacopoeial standards.
How should Cholesta-3,5-diene be handled safely?
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Cholesta-3,5-diene is classified as harmful if swallowed (H302). Handle with appropriate personal protective equipment, including a dust mask (type N95 or equivalent), eyeshields, and gloves. Ensure adequate ventilation.
How is Cholesta-3,5-diene packed and shipped?
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Cholesta-3,5-diene is stored at -20°C. We adhere to all regulations for the safe packing, handling, and export of fine chemicals.
How can I request a sample or quote for Cholesta-3,5-diene?
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To request a sample or a quotation for Cholesta-3,5-diene, please contact our sales department through the website or by calling us directly.
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