CHAPS

CAS 75621-03-3C32H58N2O7SBiochemicals & Reagents

CHAPS (CAS: 75621-03-3; C32H58N2O7S; MW: 614.88) is a zwitterionic detergent crucial in membrane biochemistry. Its non-denaturing properties make it ideal for solubilising membrane proteins and disrupting protein-protein interactions. CHAPS finds extensive use in protein purification and is particularly valuable for isoelectric focusing (IEF) and two-dimensional electrophoresis, offering excellent resolution for various biological preparations.

IUPAC: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

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CHAPS (C32H58N2O7S) — chemical structure, CAS 75621-03-3; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsCore BioreagentsDetergentsDetergents A to MResearch Essentials

01 /Applications

Membrane Protein Solubilisation

CHAPS is employed to effectively solubilise integral and peripheral membrane proteins. Its zwitterionic nature allows for the maintenance of protein structure and activity during extraction from lipid bilayers.

Protein-Protein Interaction Studies

This detergent can disrupt non-covalent protein-protein interactions, aiding in the identification and characterisation of protein complexes within cellular membranes.

Isoelectric Focusing (IEF)

CHAPS is widely used in IEF and two-dimensional electrophoresis protocols for its ability to solubilise proteins without denaturation, leading to high-resolution separation based on isoelectric point.

Electrophoresis Applications

It is suitable for both non-denaturing IEF and as an additive in gel formulations for electrophoresis, enhancing protein recovery and resolution.

02 /Properties

Molecular weight	614.88
Empirical formula	C32H58N2O7S
Assay	≥97.0%
Density	1.01 g/mL at 20 °C
Concentration	100 mM in H2O
Storage temperature	2-8°C
Application	CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS’ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

03 /Safety & handling

GHS hazard pictogram: Harmful / irritant (GHS07) — Harmful / irritant

Warning

Hazard statements

H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust, fume, gas or vapours
P305IF IN EYES

Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xi
Risk statements (R)	36/37/38
Safety statements (S)	26

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

75621-03-3

05 /Additional specifications

Mol Wt	micellar avg mol wt 6150
Availability	available only in Japan
Transition Temp	cloud point >100 °C
Aggregation Number	10
Cmc	6 mM(20-25°C)

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is CHAPS primarily used for?

CHAPS is primarily used as a non-denaturing zwitterionic detergent for membrane biochemistry, particularly for solubilising membrane proteins and in electrophoresis techniques like isoelectric focusing.

What are the CAS number and formula for CHAPS?

The CAS number for CHAPS is 75621-03-3 and its empirical formula is C32H58N2O7S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies CHAPS with an assay of ≥97.0%. This product is intended for research purposes and is not represented as USP, BP, EP, or pharmacopoeia grade.

What are the safety precautions for handling CHAPS?

CHAPS is a Warning substance that may cause skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Handle with appropriate personal protective equipment, including gloves and eye protection, and ensure adequate ventilation. Avoid breathing dust/fume/gas/mist/vapours/spray. In case of contact with eyes, rinse cautiously with water for several minutes.