Bromothymol Blue
Bromothymol Blue (CAS: 76-59-5), with the molecular formula C27H28Br2O5S, is a pH indicator (pKa ~7.1). It functions as a vital stain for biological slides, aiding in the microscopic visualisation of cell walls and nuclei. This compound is also a key component in various bacterial growth and detection media, including CLED and MacConkey agar. Tech Serve Solutions supplies Bromothymol Blue for laboratory and research applications.
- IUPAC
- 3′,3″-Dibromothymolsulfonphthalein
- Synonyms
- 3′,3″-Dibromothymolsulfonphthalein
pH Indicator
Bromothymol Blue serves as a pH indicator, changing colour around a pH of 7.1. This property makes it valuable for monitoring pH in various chemical and biological systems.
Vital Biological Stain
Utilised as a vital stain in microscopy, Bromothymol Blue helps define cellular structures such as cell walls and nuclei on biological slides, facilitating detailed observation.
Microbiological Media Component
It is incorporated into specialised bacterial growth and detection media, including Cystine Lactose Electrolyte Deficient (CLED) agar and MacConkey agar, for identifying specific microorganisms.
| Molecular weight | 624.38 |
|---|---|
| Empirical formula | C27H28Br2O5S |
| Application | Bromothymol Blue (BTB) is a pH indicator (pKa approx. 7.1) that is used as a biological slide vital stain to define cell walls or nuclei under the microscope. BTB is also a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar. |
| Melting point | 200-202 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Bromothymol Blue used for?
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Bromothymol Blue (CAS: 76-59-5) is primarily used as a pH indicator and a vital stain for microscopy. It is also a component in several microbiological growth media.
What is the CAS number and formula for Bromothymol Blue?
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The CAS number for Bromothymol Blue is 76-59-5, and its empirical formula is C27H28Br2O5S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Bromothymol Blue typically for laboratory and research use. The specific assay and purity are detailed on the product's Certificate of Analysis. We do not represent this product as USP, BP, or EP grade unless explicitly stated.
How should Bromothymol Blue be handled safely?
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Handle Bromothymol Blue with appropriate personal protective equipment, including eyeshields and gloves. A respirator filter of type N95 (US) or P1 (EN143) is recommended. Refer to the Safety Data Sheet for comprehensive handling instructions.
Is Bromothymol Blue a controlled substance?
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The provided data does not indicate that Bromothymol Blue is a controlled substance.
How is Bromothymol Blue shipped?
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Bromothymol Blue is available for export globally through Tech Serve Solutions. Packaging and shipping adhere to international regulations for chemical transport.
How can I request a sample or quote for Bromothymol Blue?
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To request a sample or a quote for Bromothymol Blue, please contact our sales team via the website or listed contact details, providing your specific requirements and location.
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