Boc-O-benzyl-Ser-Gly-Arg-p-nitroanilide
Boc-O-benzyl-Ser-Gly-Arg-p-nitroanilide (CAS 77220-80-5) is a synthetic peptide substrate. With the molecular formula C29H40N8O8, this compound is primarily utilised in biochemical research for the study of enzyme activity, particularly in relation to protease function. Its specific structure makes it a valuable tool for investigating enzymatic cleavage and reaction kinetics in vitro. Stored at −20°C, it serves as a reliable substrate in various biochemical assays.

Enzyme Activity Assays
This compound serves as a chromogenic substrate for the detection and quantification of specific enzyme activities. Upon cleavage by target enzymes, it releases p-nitroanilide, allowing for spectrophotometric measurement.
Protease Research
Utilised in the study of proteases and their mechanisms of action. Its defined peptide sequence and reporter group enable researchers to investigate enzyme specificity and inhibition.
Biochemical Reagent
A useful reagent in general biochemical research, facilitating studies on peptide bond hydrolysis and enzyme kinetics in diverse biological systems.
| Molecular weight | 628.68 |
|---|---|
| Empirical formula | C29H40N8O8 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Boc-O-benzyl-Ser-Gly-Arg-p-nitroanilide used for?
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Boc-O-benzyl-Ser-Gly-Arg-p-nitroanilide is used as a chromogenic substrate in biochemical assays to study enzyme activity, particularly for proteases.
What is the CAS number and chemical formula?
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The CAS number is 77220-80-5, and the empirical formula is C29H40N8O8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical for research purposes. We do not specify pharmacopoeia grades such as USP, BP, or EP unless explicitly stated in the product's verified data. Please refer to the Certificate of Analysis for lot-specific purity.
What are the safety and handling recommendations?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The compound has a WGK Germany rating of 3, indicating a high hazard.
How is this product packed, shipped, and exported?
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This product is packed and shipped according to standard laboratory chemical handling protocols. Tech Serve Solutions is a global exporter and can facilitate international shipments.
How can I request a sample or a quote?
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To request a sample or a quote, please contact our sales department via the website or by telephone, providing the product name and CAS number.
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