Biotinamidohexanoic acid N-hydroxysuccinimide ester
Biotinamidohexanoic acid N-hydroxysuccinimide ester (CAS: 72040-63-2), with the molecular formula C20H30N4O6S, is a chemical compound utilized in various research applications. It features a biotin moiety conjugated to a hexanoic acid spacer, with an activated N-hydroxysuccinimide (NHS) ester for efficient conjugation to primary amines. This compound is instrumental in biochemical research, particularly for protein labeling and affinity purification.
- IUPAC
- (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester,N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester,N-(+)-Biotinyl-6-aminohexanoic acid NHS ester,N-Succinimidyl N-biotinyl-6-aminocaproate,Succinimidyl 6-(biotinamido)hexanoate
- Synonyms
- N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl esterN-(+)-Biotinyl-6-aminohexanoic acid NHS esterN-Succinimidyl N-biotinyl-6-aminocaproateSuccinimidyl 6-(biotinamido)hexanoate(+)-Biotinamidocaproate N-hydroxysuccinimidyl ester
Protein Labeling and Bioconjugation
The NHS ester group readily reacts with primary amine groups on proteins, allowing for the covalent attachment of biotin. This is crucial for techniques like Western blotting, ELISA, and immunoprecipitation where biotin-mediated detection or purification is employed.
Affinity Purification
Biotinylated molecules can be purified using avidin or streptavidin affinity matrices. Biotinamidohexanoic acid N-hydroxysuccinimide ester enables the preparation of biotinylated targets for subsequent isolation and analysis.
Cell Biology Research
This reagent facilitates the study of cellular processes by enabling the specific labeling of biomolecules within or on the surface of cells. Its application aids in understanding protein localization and interactions.
Nutrition Research
As a derivative related to biotin (Vitamin B7), it may be employed in research settings investigating nutrient metabolism and transport mechanisms, although its direct role in nutrition is distinct from free biotin.
| Molecular weight | 454.54 |
|---|---|
| Empirical formula | C20H30N4O6S |
| Assay | ≥97.0% (TLC) |
| Solubility | DMF: soluble50 mg/mL, clear, faintly yellow |
| Storage temperature | −20°C |
| Melting point | 169-171 °C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Biotinamidohexanoic acid N-hydroxysuccinimide ester used for?
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Biotinamidohexanoic acid N-hydroxysuccinimide ester is primarily used for the biotinylation of molecules containing primary amines, such as proteins and peptides, facilitating subsequent detection or purification via avidin/streptavidin binding in various biochemical and cell biology research applications.
What is the CAS number and molecular formula?
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The CAS number for Biotinamidohexanoic acid N-hydroxysuccinimide ester is 72040-63-2, and its molecular formula is C20H30N4O6S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Biotinamidohexanoic acid N-hydroxysuccinimide ester with an assay of ≥97.0% (TLC). This product is intended for research purposes and is not supplied as USP, BP, EP, or pharmaceutical grade.
How should this product be handled and stored?
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Handle Biotinamidohexanoic acid N-hydroxysuccinimide ester with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 or P1 respirator filter, as it is classified under WGK Germany 3. Store the product at -20°C.
Is this chemical a controlled substance?
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The provided data does not indicate that Biotinamidohexanoic acid N-hydroxysuccinimide ester (CAS: 72040-63-2) is a controlled substance.
How is this product shipped?
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Biotinamidohexanoic acid N-hydroxysuccinimide ester is exported by Tech Serve Solutions under appropriate conditions for chemical reagents. Please contact us for specific shipping details for your region.
How can I request a sample or quote?
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To request a sample or a quote for Biotinamidohexanoic acid N-hydroxysuccinimide ester, please visit the Tech Serve Solutions website or contact our sales department directly.
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