Acridine Orange hydrochloride hydrate

CAS 65-61-2C17H19N3 · HCl · xH2OBiochemicals & Reagents

Acridine Orange hydrochloride hydrate (CAS: 65-61-2), with the formula C17H19N3 · HCl · xH2O and an anhydrous molar mass of 301.81 g/mol, is a vital fluorescent stain. It is primarily used for the detection of nucleic acids within gel electrophoresis and as a probe in the study of cellular processes such as apoptosis. Its specific spectral properties enable sensitive visualisation in biological research.

IUPAC: 3,6-Bis(dimethylamino)acridine hydrochloride

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Acridine Orange hydrochloride hydrate (C17H19N3 · HCl · xH2O) — chemical structure, CAS 65-61-2; Life Science, fine chemical supplied by Tech Serve Solutions

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01 /Applications

Nucleic Acid Staining in Gel Electrophoresis

Acridine Orange hydrochloride hydrate serves as a sensitive fluorescent stain for visualizing nucleic acids, DNA and RNA, in both agarose and polyacrylamide gels. Its ability to intercalate into nucleic acid structures allows for clear detection after electrophoresis.

Apoptosis Research

This compound has been utilised as a probe to study apoptosis, a critical process in cell biology. Its fluorescent properties facilitate the assessment of cellular changes associated with programmed cell death in various biological models.

Cell Biology Research

As a versatile fluorescent dye, Acridine Orange hydrochloride hydrate finds application in diverse cell biology studies. It aids in understanding cellular mechanisms and can be used to investigate cell cycle progression and other vital cellular functions.

02 /Properties

Molecular weight	301.81 (anhydrous basis)
Empirical formula	C17H19N3 · HCl · xH2O
Assay	99% (HPLC)
Concentration	10 mg/mL in H2O
Application	Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.
Melting point	284-287 °C(lit.)

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

65-61-2

MDL number

MFCD00150070

PubChem substance

24859123

Beilstein registry

3734978

05 /Additional specifications

Colour Index Number	46005
Absorption	λmax 492 nm

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Acridine Orange hydrochloride hydrate used for?

Acridine Orange hydrochloride hydrate is primarily used as a fluorescent stain for nucleic acids in gel electrophoresis and as a probe in apoptosis research and other cell biology studies.

What are the CAS number and formula for Acridine Orange hydrochloride hydrate?

The CAS number is 65-61-2, and the formula is C17H19N3 · HCl · xH2O.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Acridine Orange hydrochloride hydrate with an assay of 99% (HPLC). We do not represent this product as USP, BP, or EP grade.

How should Acridine Orange hydrochloride hydrate be handled safely?

Safe handling requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Observe WGK Germany classification of 3 for environmental precautions.