8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt
8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (CAS: 6358-69-6), also known as Pyranine, is a fluorescent dye with a molecular weight of 524.39 g/mol. This bright yellow to orange powder is recognised for its strong fluorescence, particularly in aqueous solutions. It is widely employed as a fluorescent indicator and probe, especially for pH-sensitive applications and in various biochemical and chemical synthesis processes.
- IUPAC
- HPTS,Pyranine,Solvent Green 7,Trisodium 8-hydroxypyrene-1,3,6-trisulfonate
- Synonyms
- 6358-69-6C16H7Na3O10S3HPTSPyranineSolvent Green 7Trisodium 8-hydroxypyrene-1,3,6-trisulfonate

Fluorescent Indicator and Probe
Pyranine serves as an effective fluorescent indicator due to its sensitivity to pH changes. Its fluorescence properties make it suitable for use as a probe in various biological and chemical studies, allowing for the visualisation and quantification of specific conditions or analytes.
Biochemical Research
In biochemical research, this compound can be utilised for labelling and tracing experiments. Its fluorescent nature aids in the detection and analysis of biological molecules and processes, contributing to a deeper understanding of cellular mechanisms.
Chemical Synthesis
As a building block and reagent, 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt is valuable in organic synthesis. It can be incorporated into more complex molecular structures or used to impart specific optical properties to synthesized compounds.
| Molecular weight | 524.39 |
|---|---|
| Empirical formula | C16H7Na3O10S3 |
| Composition | Dye content, 75% |
| Melting point | >300 °C(lit.) |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 59040 |
|---|---|
| Absorption | λmax 403 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt used for?
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This compound is primarily used as a fluorescent indicator and probe, especially for pH-sensitive applications, and in various biochemical research and chemical synthesis processes.
What are the CAS number and formula for this product?
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The CAS number is 6358-69-6, and its empirical formula (Hill notation) is C16H7Na3O10S3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with a dye content of 75%. As this is a research-grade material, TSS does not represent it as USP, BP, or EP grade.
What are the safety and handling requirements for this chemical?
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This compound is classified with GHS07 symbol and 'Warning' signal word, with hazard statements H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation). Recommended personal protective equipment includes a dust mask type N95 (US), eyeshields, and gloves. Handle with adequate ventilation and avoid dust formation.
How are your chemicals packed, shipped, and exported?
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Tech Serve Solutions has extensive experience in packing, shipping, and exporting fine chemicals globally, adhering to all relevant international regulations to ensure safe and compliant delivery.
How can I request a sample or a quote for 8-Hydroxypyrene-1,3,6-trisulfonic acid trisodium salt?
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To request a sample or a quote, please contact our sales department through the website or via email, providing your details and the product CAS number (6358-69-6).
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