6-(4-Azido-2-nitrophenylamino)hexanoic acid N-hydroxysuccinimide ester
6-(4-Azido-2-nitrophenylamino)hexanoic acid N-hydroxysuccinimide ester (CAS 64309-05-3) is a photoactive, heterobifunctional cross-linking reagent. With a molecular weight of 390.35 g/mol and formula C16H18N6O6, it is suitable for chemical biology, protein modification, and structural analysis. Its structure allows for amine-reactive coupling followed by UV-induced crosslinking.
- IUPAC
- N-Succinimidyl 6-(4-azido-2-nitroanilino)hexanoate
Amine-Reactive Crosslinking
The N-hydroxysuccinimide ester moiety reacts selectively with primary amines at pH 6.5-8.5, forming stable amide bonds. This enables conjugation to proteins, peptides, and other amine-containing biomolecules.
Photoactivated Crosslinking
Upon UV irradiation (250-350 nm), the azido group generates a reactive nitrene intermediate. This highly reactive species can crosslink to nearby molecules, facilitating studies of molecular interactions and protein structural mapping.
Bioconjugation Chemistry
This reagent serves as a valuable tool in bioconjugation, enabling the creation of complex molecular assemblies. Its heterobifunctional nature allows for stepwise or directed conjugation strategies in various biochemical research applications.
| Molecular weight | 390.35 |
|---|---|
| Empirical formula | C16H18N6O6 |
| Assay | ≥85% |
| Form | powder |
| Colour | orange |
| Solubility | ethyl acetate: soluble10 mg/mLDMF: soluble |
| Storage temperature | −20°C |
| Application | Photoactive, heterobifunctional cross-linking reagent incorporating an extended spacer. Typically, initial reaction couples via ester to primary amine by amide bond formation in the pH range 6.5-8.5. Second bonding occurs during UV irradiation (250-350 nm) via reactive nitrene. The latter bonding is rapid and non-specific. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 6-(4-Azido-2-nitrophenylamino)hexanoic acid N-hydroxysuccinimide ester used for?
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It is used as a photoactive, heterobifunctional cross-linking reagent in chemical biology and protein modification studies. It can react with primary amines and subsequently undergo UV-induced crosslinking.
What are the CAS number and formula for this compound?
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The CAS number is 64309-05-3, and the molecular formula is C16H18N6O6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical with an assay of ≥85%. This product is intended for research and laboratory use and is not supplied as USP, BP, EP, or pharmacopoeia grade.
How should this chemical be handled safely?
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Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 or P1 respirator filter, should be used. Refer to the safety data sheet for comprehensive handling information.
Is this product a controlled substance?
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The provided data does not indicate that this chemical is a controlled substance.
How is this product packed, shipped, and exported?
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This chemical is typically supplied as an orange powder and stored at -20°C. Shipping and export are handled in accordance with relevant regulations for chemical compounds.
How can I request a sample or quote for this product?
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To request a sample or a quote, please contact the Tech Serve Solutions sales team through our website or by phone.
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