5,5′-Dithiobis(2-nitrobenzoic acid)
5,5′-Dithiobis(2-nitrobenzoic acid) (CAS 69-78-3), with a molecular weight of 396.35, is a valuable organic compound. It is characterized by its disulfide bond and nitrobenzoic acid functionalities. Commonly known as DTNB or Ellman's Reagent, it serves as a crucial reagent in biochemical assays for quantifying sulfhydryl (thiol) groups in proteins and other molecules.
- IUPAC
- 3-Carboxy-4-nitrophenyl disulfide,6,6′-Dinitro-3,3′-dithiodibenzoic acid,Bis(3-carboxy-4-nitrophenyl) disulfide,DTNB,Ellman’s Reagent
- Synonyms
- [-SC6H3(NO2)CO2H]23-Carboxy-4-nitrophenyl disulfide6,6′-Dinitro-3,3′-dithiodibenzoic acidBis(3-carboxy-4-nitrophenyl) disulfideDTNBEllman’s Reagent
![5,5′-Dithiobis(2-nitrobenzoic acid) ([-SC6H3(NO2)CO2H]2) — chemical structure, CAS 69-78-3; Chemistry, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F69-78-3.png&w=3840&q=75)
Biochemical Assays
Utilised as Ellman's Reagent, this compound is essential for the quantitative determination of free sulfhydryl groups in biological samples. It reacts with thiols to produce a coloured product, allowing for spectrophotometric analysis.
Protein Chemistry
In protein research, DTNB is employed to study protein structure and function by detecting and quantifying cysteine residues. This aids in understanding protein folding, disulfide bond formation, and enzyme activity.
Chemical Synthesis
As a building block, 5,5′-Dithiobis(2-nitrobenzoic acid) can be incorporated into more complex organic molecules. Its reactive disulfide and carboxylic acid groups offer versatility in synthetic organic chemistry.
| Molecular weight | 396.35 |
|---|---|
| Linear formula | [-SC6H3(NO2)CO2H]2 |
| Assay | 99% |
| Grade | ReagentPlus® |
| Solubility | Do you have solubility information on this product that you would like to share? |
| Suitability | suitable for determination of sulfhydryl groups |
| Melting point | 240-245 °C (dec.)(lit.) |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5,5′-Dithiobis(2-nitrobenzoic acid) primarily used for?
+
5,5′-Dithiobis(2-nitrobenzoic acid) is primarily used as Ellman's Reagent for the quantitative determination of sulfhydryl (thiol) groups in biochemical and protein chemistry applications.
What is the CAS number and molecular formula for this compound?
+
The CAS number is 69-78-3. The linear formula is [-SC₆H₃(NO₂)CO₂H]₂.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this product at a ReagentPlus® grade with an assay of 99% purity. It is suitable for determination of sulfhydryl groups. We do not represent it as USP, BP, or EP grade.
How should 5,5′-Dithiobis(2-nitrobenzoic acid) be handled safely?
+
This compound is classified with GHS07 warning symbol, indicating it can cause skin irritation (H315), eye irritation (H319), and respiratory irritation (H335). Appropriate personal protective equipment, including a dust mask type N95 (US), eyeshields, and gloves, should be used during handling. Ensure adequate ventilation.
Is this product available for export?
+
Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including 5,5′-Dithiobis(2-nitrobenzoic acid).
How can I request a sample or quote?
+
Please contact our sales team directly through our website or by telephone to request a sample or a quotation for 5,5′-Dithiobis(2-nitrobenzoic acid).
▶ Related products
1-(2-Fluorobenzyl)piperazine
C11H15FN2
Chemical Synthesis
1-(2-Fluorophenyl)biguanide hydrochloride
Chemical Synthesis
1-(2-Fluorophenyl)cyclopropanecarboxylic acid
C10H9FO2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine
C10H13FN2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine monohydrochloride
C10H13FN2 · HCl
Chemical Synthesis
1-(2-Furoyl)-1H-benzotriazole
C11H7N3O2
Chemical Synthesis
1-(2-Furoyl)piperazine
C9H12N2O2
Chemical Synthesis
1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione
Chemical Synthesis
▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis