α-(4-Pyridyl N-oxide)-N-tert-butylnitrone

CAS 66893-81-0C10H14N2O2Chemical Synthesis

α-(4-Pyridyl N-oxide)-N-tert-butylnitrone (CAS: 66893-81-0), a heterocyclic organic compound with the formula C10H14N2O2 and a molecular weight of 194.23 g/mol, serves as a crucial spin trap reagent. Its primary application lies in Electron Spin Resonance (ESR/EPR) spectroscopy for detecting and quantifying free radicals. This compound is instrumental in studies involving oxidative processes, radical formation in biological systems, and chemical synthesis.

IUPAC: N-tert-Butyl-α-(4-pyridyl)nitrone N′-oxide,N-(4-Pyridylmethylene)-tert-butylamine N,N′-dioxide,POBN

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α-(4-Pyridyl N-oxide)-N-tert-butylnitrone (C10H14N2O2) — chemical structure, CAS 66893-81-0; Chemistry, fine chemical supplied by Tech Serve Solutions

Analytical ReagentsAnalytical/ChromatographyBuilding BlocksC10Chemical SynthesisHeterocyclic Building BlocksPyridinesSpectroscopy

01 /Applications

Spin Trapping Reagent

This nitrone derivative functions as a spin trap in Electron Spin Resonance (ESR/EPR) spectroscopy. It reacts with transient free radicals to form more stable nitroxide radicals, which can then be detected and analysed, enabling the study of radical species in various chemical and biological systems.

Ethanol Oxidation Studies

It is specifically used to measure the oxidation of ethanol to the 1-hydroxyethyl radical (HER). This application is vital for understanding ethanol metabolism and its associated biological impacts, including protein binding studies.

Free Radical Detection in Biological Systems

The compound has been employed to detect free-radical formation in various biological contexts, such as DNA methylation, iron-mediated lipid peroxidation, and the generation of radicals by Alzheimer's β-amyloid peptide. It has also been used to detect hydroxyl radical formation in chondrocytes and cartilage.

Chemical Synthesis Building Block

As a heterocyclic compound containing pyridine and nitrone functionalities, it can serve as a versatile building block in organic synthesis for the creation of more complex molecules.

02 /Properties

Molecular weight	194.23
Empirical formula	C10H14N2O2
Assay	99%
Solubility	water: soluble10 mg/mL, clear, colorless to faintly yellow
Application	Spin trap reagent used to measure ethanol oxidation to 1-hydroxyethyl radical (HER) which can bind to protein. Has also been used in studies of DNA methylation, iron-mediated polyunsaturated fatty acid peroxidation, and free-radical formation by Alzheimer β-amyloid peptide. The formation of hydroxyl radical in chondrocytes and cartilage has been detected using POBN.
Melting point	183-185 °C(lit.)

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

66893-81-0

MDL number

MFCD00041022

PubChem substance

24852894

Beilstein registry

1453764

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is α-(4-Pyridyl N-oxide)-N-tert-butylnitrone used for?

α-(4-Pyridyl N-oxide)-N-tert-butylnitrone is primarily used as a spin trap reagent in Electron Spin Resonance (ESR/EPR) spectroscopy for the detection and study of free radicals in chemical and biological systems. It is also utilised in research concerning ethanol oxidation, DNA methylation, lipid peroxidation, and radical formation by amyloid peptides.

What is the CAS number and molecular formula?

The CAS number is 66893-81-0, and the molecular formula is C10H14N2O2.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this compound with an assay of 99%. We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.

What are the safety and handling recommendations for this product?

When handling α-(4-Pyridyl N-oxide)-N-tert-butylnitrone, it is essential to wear eyeshields and gloves. A respirator filter, such as type N95 (US) or type P1 (EN143), should be used. The compound is classified under WGK Germany 3, indicating a significant hazard to water.

How is this chemical packed, shipped, and exported?

Tech Serve Solutions packages and exports chemicals in compliance with international shipping regulations, ensuring safe and secure delivery to customers worldwide. Specific packaging details are available upon request.

How can I request a sample or quote?

To request a sample or a quote for α-(4-Pyridyl N-oxide)-N-tert-butylnitrone, please contact our sales department through the website or by phone, providing your contact details and the quantity required.