4-Nitroazobenzene
4-Nitroazobenzene (CAS 2491-52-3), with the molecular formula C6H5N=NC6H4NO2 and a molecular weight of 227.22 g/mol, is a chemical compound identified by the MDL number MFCD00007309. This nitro dye is primarily utilised in staining applications within cell biology and histology. Its chemical structure makes it a useful reagent in various laboratory settings for analytical and research purposes.
- IUPAC
- NAB

Histological Staining
4-Nitroazobenzene serves as a staining agent in histology, aiding in the microscopic visualisation of tissue samples. Its properties allow for differential staining, enhancing the identification of cellular structures.
Cell Biology Reagent
In cell biology, this compound can be employed as a laboratory reagent for specific experimental procedures. Its application is often related to cell structure analysis or as a component in staining protocols.
Dye and Pigment Research
As a nitro dye, 4-Nitroazobenzene may find use in research concerning the development or analysis of dyes and pigments. Its azobenzene structure is a common chromophore in dye chemistry.
| Molecular weight | 227.22 |
|---|---|
| Linear formula | C6H5N=NC6H4NO2 |
| Assay | 90% |
| Melting point | 132-134 °C(lit.) |


Hazard statements
- H302Harmful if swallowed
- H312Harmful in contact with skin
- H315Causes skin irritation
- H319Causes serious eye irritation
- H332Harmful if inhaled
- H335May cause respiratory irritation
- H351Suspected of causing cancer
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/21/22-36/37/38-40 |
| Safety statements (S) | 26-27-36/37/39 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitroazobenzene used for?
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4-Nitroazobenzene is used in histology and cell biology as a staining agent and laboratory reagent for microscopic analysis and research.
What is the CAS number and molecular formula for 4-Nitroazobenzene?
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The CAS number for 4-Nitroazobenzene is 2491-52-3, and its molecular formula is C6H5N=NC6H4NO2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Nitroazobenzene with an assay of 90%. This product is not classified as USP, BP, EP, or other pharmacopoeial grades.
How should 4-Nitroazobenzene be handled safely?
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Handling requires appropriate personal protective equipment, including eyeshields, gloves, and respiratory protection (e.g., type N100 respirator). Avoid breathing dust/fume/gas/mist/vapours/spray and ensure adequate ventilation. Refer to the safety data sheet for comprehensive handling instructions and hazard information.
Is 4-Nitroazobenzene a controlled substance?
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The provided data does not indicate that 4-Nitroazobenzene is a controlled substance.
How is 4-Nitroazobenzene packed and shipped?
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Tech Serve Solutions exports fine chemicals globally. Specific packaging and shipping details are provided upon request to comply with all relevant regulations.
How can I request a quote or sample for 4-Nitroazobenzene?
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To request a quote or sample of 4-Nitroazobenzene, please contact the Tech Serve Solutions sales team through our website or by phone.
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