4-Amino-1,1′-azobenzene-3,4′-disulfonic acid monosodium salt
4-Amino-1,1′-azobenzene-3,4′-disulfonic acid monosodium salt (CAS 74543-21-8) is an azo dye and a disulfonic acid derivative. With a molecular weight of 379.34 g/mol, it exhibits an absorption maximum at 489 nm. This compound is primarily utilized as a dye in biological and histological staining applications, contributing to cell visualization and tissue differentiation in research and diagnostic settings.
- IUPAC
- 2-Amino-5-([4-sulfophenyl]azo)benzenesulfonic acid monosodium salt,Acid Yellow 9 monosodium salt
- Synonyms
- 74543-21-8RO3SC6H4N=NC6H3(NH2)SO3R, R = H or Na2-Amino-5-([4-sulfophenyl]azo)benzenesulfonic acid monosodium saltAcid Yellow 9 monosodium salt

Biological Staining
This compound serves as a key dye in cell biology, aiding in the visualization and differentiation of cellular structures. Its specific spectral properties allow for clear observation under microscopy.
Histological Staining
In hematology and histology, it is employed for staining tissue samples. This application is crucial for identifying various cell types and pathological changes in medical diagnostics and research.
Dye Applications
As a member of the stains and dyes category, it is suitable for various laboratory and research purposes where a specific colorant is required. Its precise chemical structure ensures reliable performance.
| Molecular weight | 379.34 |
|---|---|
| Linear formula | RO3SC6H4N=NC6H3(NH2)SO3R, R = H or Na |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
Precautionary statements
- P305IF IN EYES
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 489 nm |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Amino-1,1′-azobenzene-3,4′-disulfonic acid monosodium salt used for?
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This compound is primarily used as a dye in cell biology, hematology, and histology for staining and visualization purposes.
What are the CAS number and formula for this chemical?
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The CAS number is 74543-21-8. The linear formula is RO₃SC₆H₄N=NC₆H₃(NH₂)SO₃R, where R can be H or Na. The molecular weight is 379.34 g/mol.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical for research and laboratory use. The exact grade and purity are specified in the product documentation. We do not represent this product as USP, BP, or EP grade unless explicitly stated.
How should this chemical be handled safely?
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This compound is classified as a warning substance, causing skin and eye irritation (H315, H319). Handle with appropriate personal protective equipment, including gloves and eye protection, and ensure good ventilation. Avoid contact with skin and eyes. In case of contact, rinse immediately with plenty of water and seek medical advice. Refer to the Safety Data Sheet (SDS) for comprehensive safety information.
Is this product available for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including this product, serving customers worldwide.
How can I request a sample or quote?
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Please contact our sales team via the website or phone to request a sample or a quotation for 4-Amino-1,1′-azobenzene-3,4′-disulfonic acid monosodium salt.
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