4′,6-Diamidino-2-phenylindole dihydrochloride
4′,6-Diamidino-2-phenylindole dihydrochloride (CAS 28718-90-3), molecular formula C16H15N5 · 2HCl, is a high-purity chemical supplied by Tech Serve Solutions for biochemicals & reagents applications, to pharmacopoeia specification (USP, BP, EP). Every batch is tested and ships with a Certificate of Analysis; MSDS and technical data sheets are available on request. Tech Serve Solutions has supplied laboratory reagents, fine chemicals and pharmaceutical intermediates worldwide since 1998.
- IUPAC
- 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride,DAPI dihydrochloride

| Molecular weight | 350.25 |
|---|---|
| Empirical formula | C16H15N5 · 2HCl |
| Assay | ≥98% (HPLC and TLC) |
| Solubility | H2O: soluble20 mg/mL (Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)PBS: insoluble |
| Storage temperature | 2-8°C |
| Application | DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma. |
| Suitability | suitable for cell culturesuitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Absorption | ε/263 nm, H2O 30 |
| Fluorescence | λex 340 nm; λem 488 nm (nur DAPI)λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) |
Documentation
Supplied to USP, BP and EP standards. Every batch ships with a Certificate of Analysis (assay, identity, purity); MSDS and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4′,6-Diamidino-2-phenylindole dihydrochloride?
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4′,6-Diamidino-2-phenylindole dihydrochloride (CAS 28718-90-3), molecular formula C16H15N5 · 2HCl, is a high-purity chemical supplied by Tech Serve Solutions for biochemicals & reagents applications, to USP, BP and EP standards.
What grades of 4′,6-Diamidino-2-phenylindole dihydrochloride does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4′,6-Diamidino-2-phenylindole dihydrochloride in USP, BP, EP and reagent/AR grades. Custom specifications for nutraceutical or specialty pharmaceutical applications are available — contact our technical sales team.
What is the CAS number and molecular formula of 4′,6-Diamidino-2-phenylindole dihydrochloride?
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4′,6-Diamidino-2-phenylindole dihydrochloride has CAS number 28718-90-3 and molecular formula C16H15N5 · 2HCl. Full specifications appear in the table above and in the Certificate of Analysis.
What is the typical minimum order quantity and lead time for 4′,6-Diamidino-2-phenylindole dihydrochloride?
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Sample quantities are available for technical evaluation; bulk MOQ depends on grade and packaging. In-stock material typically ships in 7–10 working days; custom-spec orders take 3–4 weeks. Tech Serve Solutions ships worldwide with full export documentation.
Do you provide a Certificate of Analysis (CoA) with each shipment?
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Yes. Every batch of 4′,6-Diamidino-2-phenylindole dihydrochloride ships with a Certificate of Analysis covering assay, identity and purity tests, plus pharmacopoeia conformance where applicable. MSDS and TDS documents are available on request before order placement.
How should 4′,6-Diamidino-2-phenylindole dihydrochloride be stored?
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Store 4′,6-Diamidino-2-phenylindole dihydrochloride in its original sealed container in a cool, dry place away from direct sunlight and incompatible materials, in line with the storage guidance on the Safety Data Sheet. Reseal between dispensings.
What is 4′,6-Diamidino-2-phenylindole dihydrochloride used for?
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4′,6-Diamidino-2-phenylindole dihydrochloride is used in biochemicals & reagents applications across pharmaceutical, research and industrial settings. Our technical team can advise on suitability for your specific process.
How do I request a quote for 4′,6-Diamidino-2-phenylindole dihydrochloride?
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Request a quote with your required grade, quantity and destination via https://www.techservesolutions.in/contact or email info@techservesolutions.in. We respond with specifications, a Certificate of Analysis and pricing.
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