4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride
4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride (CAS: 81864-15-5) is a fluorogenic reagent with a molecular weight of 225.07 g/mol. This compound, supplied as a powder, is primarily utilised in biochemical research for the derivatisation of alpha-ketoacids. Its solubility in water (50 mg/mL) facilitates its use in various laboratory applications. Stored at -20°C, this chemical is essential for sensitive analytical procedures.
- IUPAC
- 1,2-Diamino-4,5-methylenedioxybenzene dihydrochloride,1,3-Benzodioxole-5,6-diamine dihydrochloride,5,6-Diamino-1,3-benzodioxole dihydrochloride,MDB
- Synonyms
- 81864-15-5C7H8N2O2 · 2HCl1,2-Diamino-4,5-methylenedioxybenzene dihydrochloride1,3-Benzodioxole-5,6-diamine dihydrochloride5,6-Diamino-1,3-benzodioxole dihydrochlorideMDB
Fluorogenic Derivatisation Reagent
Utilised for the sensitive detection and quantification of alpha-ketoacids. The reaction of 4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride with alpha-ketoacids produces fluorescent derivatives that can be easily measured using fluorometry.
Biochemical Analysis
Serves as a valuable tool in biochemical studies, particularly in metabolomics and enzymatic assays. It aids in identifying and measuring key metabolic intermediates.
Histology and Cell Biology Research
Its properties make it suitable for use in histological staining and cell biology research, potentially as a detection or labelling agent within these fields.
| Molecular weight | 225.07 |
|---|---|
| Empirical formula | C7H8N2O2 · 2HCl |
| Assay | ≥98% (HPLC) |
| Form | powder |
| Solubility | H2O: soluble50 mg/mL |
| Storage temperature | −20°C |
| Application | Fluorogenic reagent for α-ketoacids. |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride used for?
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This compound is primarily used as a fluorogenic reagent for the derivatisation of alpha-ketoacids, enabling their sensitive detection and quantification in biochemical analyses.
What are the CAS number and molecular formula?
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The CAS number is 81864-15-5, and the molecular formula is C7H8N2O2 · 2HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥98% (HPLC). It is intended for research and laboratory use and is not represented as USP, BP, EP, or pharmaceutical grade.
How should this chemical be handled safely?
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This compound is a warning-level substance (GHS07) that may cause skin irritation (H315), eye irritation (H319), and respiratory irritation (H335). Appropriate personal protective equipment, including a dust mask (N95), eyeshields, and gloves, should be worn. Ensure adequate ventilation.
Is this product shipped internationally?
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Yes, Tech Serve Solutions specialises in supplying and exporting fine chemicals globally to research institutions and commercial entities.
How can I request a sample or quote?
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To request a sample or a quotation, please contact our sales department via the website or direct email, providing the product name and CAS number (81864-15-5).
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