4(5)-(Iodoacetamido)fluorescein
4(5)-(Iodoacetamido)fluorescein (CAS: 63368-54-7, C22H14INO6) is a fluorescent labeling reagent with a molecular weight of 515.25. This compound, also known by the abbreviation 5-IAF, is primarily utilised in biochemical research for covalently labelling proteins and other biomolecules via reaction with thiol groups. Its inherent fluorescence allows for sensitive detection and quantification in various biological assays and imaging applications.
- IUPAC
- 5-IAF
Protein Labelling
4(5)-(Iodoacetamido)fluorescein is employed to covalently attach a fluorescent tag to proteins, enabling their detection and tracking in biological systems. The iodoacetamide group reacts specifically with cysteine residues.
Fluorescent Probes
As a derivative of fluorescein, this compound serves as a fluorescent probe. It can be used in fluorescence microscopy and flow cytometry to visualise cellular structures or quantify cellular components.
Biochemical Assays
It is a valuable reagent in various biochemical assays where a fluorescent readout is required, such as enzyme activity measurements or ligand binding studies, by labelling key biomolecules.
| Molecular weight | 515.25 |
|---|---|
| Empirical formula | C22H14INO6 |
| Assay | ≥80.0% (HPLC) |
| Storage temperature | −20°C |
| Composition | Dye content, 90% |
| Melting point | 265-267 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 266 nm in DMF |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4(5)-(Iodoacetamido)fluorescein used for?
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4(5)-(Iodoacetamido)fluorescein is primarily used for covalently labelling proteins and other biomolecules, particularly reacting with thiol groups, for use in fluorescent detection, assays, and imaging.
What is the CAS number and chemical formula for 4(5)-(Iodoacetamido)fluorescein?
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The CAS number is 63368-54-7 and the empirical formula is C22H14INO6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4(5)-(Iodoacetamido)fluorescein with an assay of ≥80.0% (HPLC) and dye content of 90%. We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should 4(5)-(Iodoacetamido)fluorescein be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a P1 or N95 respirator. Store at -20°C. This compound is classified under WGK Germany 3, indicating it is hazardous to water.
How is this product packed, shipped, and exported?
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Tech Serve Solutions, a global supplier and exporter since 1998, ensures appropriate packaging for shipping and export of fine chemicals like 4(5)-(Iodoacetamido)fluorescein.
How can I request a quote or sample?
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To request a quote or sample of 4(5)-(Iodoacetamido)fluorescein, please contact our sales team through the website or by phone.
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