3-Phenylumbelliferone
3-Phenylumbelliferone (CAS: 6468-96-8, C15H10O3) is a fluorescent compound with a molecular weight of 238.24 g/mol. It is characterised by its utility as a pH indicator and for its fluorescent properties. This compound is soluble in DMF, DMSO, and alcohols, making it versatile for various laboratory applications. Available from Tech Serve Solutions, it serves as a valuable reagent in chemical and biological research, particularly in detection and cell biology studies.
- IUPAC
- 7-Hydroxy-3-phenylcoumarin

pH Indicator
3-Phenylumbelliferone functions as a pH indicator due to its distinct fluorescence changes across different pH levels. Its spectral properties shift significantly, allowing for visual or instrumental detection of pH variations in solutions.
Fluorescent Probe
With excitation and emission maxima at specific wavelengths (e.g., λex 338 nm; λem 463 nm at pH 3.0), this compound is suitable for fluorescence-based assays. It can be employed in studies requiring sensitive detection of biological or chemical processes.
Biochemical Research
As a bioactive small molecule, 3-Phenylumbelliferone finds application in biochemical research. It is used in studies involving cell biology and detection methods, contributing to the understanding of various biological mechanisms.
| Molecular weight | 238.24 |
|---|---|
| Empirical formula | C15H10O3 |
| Assay | ≥98.0% (TLC) |
| Solubility | DMF: solubleDMSO: solublealcohols: soluble |
| Application | suitable as pH-indicator |
| Suitability | suitable for fluorescence |
| Melting point | 206-210 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Pka  | 7.8 |
|---|---|
| Gene Information | human ... MIF(4282) |
| Fluorescence | λex 338 nm; λem 463 nm (pH 3.0)λex 383 nm; λem 472 nm in 0.1 M Tris pH 9.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3-Phenylumbelliferone used for?
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3-Phenylumbelliferone is primarily used as a pH indicator and as a fluorescent probe in various detection and biochemical research applications, including cell biology studies.
What are the CAS number and molecular formula for 3-Phenylumbelliferone?
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The CAS number for 3-Phenylumbelliferone is 6468-96-8, and its molecular formula is C15H10O3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 3-Phenylumbelliferone with an assay of ≥98.0% (TLC). We do not represent this product as USP, BP, EP, or other pharmacopoeia grades.
What are the safety precautions for handling 3-Phenylumbelliferone?
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Handling 3-Phenylumbelliferone requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, due to its classification under WGK Germany 3.
How is 3-Phenylumbelliferone packed, shipped, and exported?
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3-Phenylumbelliferone is packed and shipped in compliance with relevant regulations for chemical substances. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals.
How can I request a sample or quote for 3-Phenylumbelliferone?
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To request a sample or quote for 3-Phenylumbelliferone, please contact our sales team through the TSS website or your dedicated account manager.
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