3-Nitrotetrazolium blue chloride

CAS 127615-60-5C40H30Cl2N10O6Biochemicals & Reagents

3-Nitrotetrazolium blue chloride (CAS: 127615-60-5) is a complex organic salt with the chemical formula C40H30Cl2N10O6 and a molecular weight of 817.64 g/mol. This tetrazolium salt serves as a chromogenic substrate, particularly useful in biochemical assays. It is employed in research and diagnostics to detect enzymatic activity, often yielding a coloured precipitate upon reduction.

IUPAC: 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[5-(3-nitrophenyl)-2-phenyl-2H-tetrazolium chloride]

Email us

3-Nitrotetrazolium blue chloride (C40H30Cl2N10O6) — chemical structure, CAS 127615-60-5; Life Science, fine chemical supplied by Tech Serve Solutions

Alkaline PhosphataseBiochemicals and ReagentsCytokinin dehydrogenaseEnzyme SubstratesEnzymesInhibitorsand SubstratesFuroyl-CoA dehydrogenaseGlucose OxidaseLactic Dehydrogenase

01 /Applications

Chromogenic Substrate for Enzyme Assays

3-Nitrotetrazolium blue chloride is widely used as a chromogenic substrate in enzymatic reactions. It is reduced by various enzymes, leading to the formation of a coloured formazan product, facilitating spectrophotometric or visual detection of enzyme activity.

Biochemical Research

This compound is valuable in biochemical research for studying enzyme kinetics and mechanisms. Its ability to form a distinct colour upon reduction allows for quantitative analysis in various biological systems and experimental setups.

Diagnostic Reagent

In diagnostic applications, it can be incorporated into assay kits for the detection of specific enzyme activities relevant to disease states. The resulting colour change provides a clear indication of the presence or level of enzyme activity.

02 /Properties

Molecular weight	817.64
Empirical formula	C40H30Cl2N10O6
Assay	≥75% (AT)
Storage temperature	2-8°C

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

127615-60-5

MDL number

MFCD01457174

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 3-Nitrotetrazolium blue chloride used for?

3-Nitrotetrazolium blue chloride is primarily used as a chromogenic substrate in biochemical assays to detect enzyme activity, often yielding a coloured precipitate upon reduction. It is valuable in biochemical research and diagnostic applications.

What is the CAS number and chemical formula for 3-Nitrotetrazolium blue chloride?

The CAS number for 3-Nitrotetrazolium blue chloride is 127615-60-5, and its chemical formula is C40H30Cl2N10O6.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies 3-Nitrotetrazolium blue chloride with an assay of ≥75% (AT). This product is intended for laboratory and research use and is not represented as USP, BP, EP, or pharmacopoeia grade.

What are the safety and handling requirements for 3-Nitrotetrazolium blue chloride?

For safe handling, it is essential to use eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used. This compound is classified with a WGK (Germany) of 3, indicating it is hazardous to water.

How is 3-Nitrotetrazolium blue chloride stored and shipped?

3-Nitrotetrazolium blue chloride should be stored at 2-8°C. Tech Serve Solutions can export this chemical globally, adhering to all necessary shipping regulations.