3,3′-Diaminobenzidine tetrahydrochloride hydrate
3,3′-Diaminobenzidine tetrahydrochloride hydrate (CAS: 868272-85-9) is a white to off-white powder supplied by Tech Serve Solutions. With a molecular weight of 360.11 on an anhydrous basis, this compound serves as a critical reagent in biochemical research and diagnostics. Its primary applications include use as a chromogenic substrate for peroxidase-based detection systems in techniques such as immunohistochemistry and Western blotting, contributing to sensitive and specific signal amplification.
- Synonyms
- 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride hydrate
Chromogenic Substrate
3,3′-Diaminobenzidine tetrahydrochloride hydrate is widely used as a chromogenic substrate for peroxidase enzymes. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, it undergoes oxidation to form a coloured precipitate, enabling visual detection.
Immunohistochemistry (IHC)
This compound is a staple in IHC staining protocols. It reacts with peroxidase-labelled antibodies to produce a distinct, insoluble brown precipitate at the site of antigen localisation, facilitating precise tissue analysis.
Western Blotting
In Western blotting, 3,3′-Diaminobenzidine tetrahydrochloride hydrate is employed as a substrate for HRP-conjugated secondary antibodies. The resulting coloured band indicates the presence and location of specific proteins on a membrane.
Enzyme-Linked Detection Assays
Beyond IHC and Western blotting, it is also applicable in other enzyme-linked detection methods where peroxidase activity needs to be visualised, offering a sensitive means of quantifying or localising target molecules.
| Molecular weight | 360.11 (anhydrous basis) |
|---|---|
| Empirical formula | C12H14N4 · 4HCl · xH2O |
| Assay | ≥96% |
| Form | powder |
| Solubility | H2O: soluble50 mg/mL |
Hazard statements
- H302Harmful if swallowed
- H319Causes serious eye irritation
- H341Suspected of causing genetic defects
- H350May cause cancer
Precautionary statements
- P201Obtain special instructions before use
- P281Use personal protective equipment as required
- P305IF IN EYES
- P308IF exposed or concerned
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 45-22-36-68 |
| Safety statements (S) | 53-26-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3,3′-Diaminobenzidine tetrahydrochloride hydrate used for?
+
This compound is primarily used as a chromogenic substrate for peroxidase enzymes in various biochemical and diagnostic applications, including immunohistochemistry and Western blotting, to visualise the presence of specific antigens or proteins.
What is the CAS number and formula for 3,3′-Diaminobenzidine tetrahydrochloride hydrate?
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The CAS number is 868272-85-9. The empirical formula is C12H14N4 · 4HCl · xH2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 3,3′-Diaminobenzidine tetrahydrochloride hydrate with an assay of ≥96%. This product is not represented as USP, BP, EP, or any other pharmacopoeia grade.
How should 3,3′-Diaminobenzidine tetrahydrochloride hydrate be handled safely?
+
This compound is harmful if swallowed, causes serious eye irritation, is suspected of causing genetic defects, and may cause cancer. Handle with appropriate personal protective equipment, including eyeshields, gloves, and a suitable respirator. Ensure adequate ventilation and avoid exposure.
How is 3,3′-Diaminobenzidine tetrahydrochloride hydrate packed and shipped?
+
3,3′-Diaminobenzidine tetrahydrochloride hydrate is supplied as a powder and is available for export globally by Tech Serve Solutions. Specific packaging details are available upon request.
How can I request a sample or quote for 3,3′-Diaminobenzidine tetrahydrochloride hydrate?
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To request a sample or a quote for 3,3′-Diaminobenzidine tetrahydrochloride hydrate, please contact our sales team through the TSS website or by calling our office.
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