3,3′-Diaminobenzidine
3,3′-Diaminobenzidine (CAS 91-95-2) is an organic compound with the linear formula (C6H3(NH2)2)2. It is a key reagent with a molecular weight of 214.27 g/mol. Primarily recognised for its role as a peroxidase substrate in biochemical assays, it also serves as a reagent for the spectrophotometric determination of selenium. Supplied by Tech Serve Solutions with an assay of ≥98.0% (CHN).
- IUPAC
- 3,3′,4,4′-Biphenyltetramine,3,3′,4,4′-Tetraaminobiphenyl,DAB
- Synonyms
- 3,3′,4,4′-Biphenyltetramine3,3′,4,4′-TetraaminobiphenylDAB
Peroxidase Substrate
3,3′-Diaminobenzidine is widely employed as a chromogenic substrate for peroxidases in various biochemical and histochemical staining techniques. It reacts with peroxidase enzymes to produce a coloured precipitate, enabling visualisation of enzyme activity.
Selenium Determination
This compound serves as a critical reagent in the spectrophotometric analysis of selenium. Its reaction with selenium allows for accurate quantitative determination of this element in various samples.
Chemical Synthesis
As an organic building block, 3,3′-Diaminobenzidine is utilized in the synthesis of more complex organic molecules and heterocyclic compounds, contributing to diverse chemical research and development.
| Molecular weight | 214.27 |
|---|---|
| Linear formula | (NH2)2C6H3C6H3(NH2)2 |
| Assay | ≥98.0% (CHN) |
| Solubility | dioxane: soluble0.1 g/10 mL, clear, colorless |
| Storage temperature | 2-8°C |
| Application | Peroxidase substrate; reagent for spectrophotometric determination of selenium. |
| Melting point | 173-176 °C175-177 °C(lit.) |
Hazard statements
- H341Suspected of causing genetic defects
- H350May cause cancer
Precautionary statements
- P201Obtain special instructions before use
- P281Use personal protective equipment as required
- P308IF exposed or concerned
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 1 |
| Hazard codes (EU) | T |
| Risk statements (R) | 45-68 |
| Safety statements (S) | 53-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3,3′-Diaminobenzidine used for?
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3,3′-Diaminobenzidine is primarily used as a peroxidase substrate in biochemical assays and as a reagent for the spectrophotometric determination of selenium. It also functions as a building block in chemical synthesis.
What is the CAS number and formula for 3,3′-Diaminobenzidine?
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The CAS number for 3,3′-Diaminobenzidine is 91-95-2, and its linear formula is (NH2)2C6H3C6H3(NH2)2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 3,3′-Diaminobenzidine with an assay of ≥98.0% (CHN). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety concerns when handling 3,3′-Diaminobenzidine?
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3,3′-Diaminobenzidine is classified as Danger (GHS08) with hazards H341 (Suspected of causing genetic defects) and H350 (May cause cancer). Appropriate personal protective equipment, including a dust mask type N95 (US), eyeshields, and gloves, must be used. Handle with extreme caution in a well-ventilated area.
How is 3,3′-Diaminobenzidine packed and shipped?
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3,3′-Diaminobenzidine is stored at 2-8°C. Specific packaging and shipping details for export are available upon request. Please contact TSS for further information.
How can I request a sample or quote for 3,3′-Diaminobenzidine?
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To request a sample or quote for 3,3′-Diaminobenzidine, please contact our sales team directly through the website or by phone. We are happy to assist with your chemical needs.
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